IKZF1 Antibody / Lymphoid Development Transcription Factor Antibody (R32214)

IKZF1 Antibody MCF-7 IF. Immunofluorescent staining of MCF-7 cells using IKZF1 Antibody / Ikaros Family Zinc Finger 1 Antibody demonstrates strong green fluorescence within the cellular compartment, consistent with expression of IKZF1 (Ikaros), a zinc finger transcription factor that regulates hematopoietic differentiation, lymphoid development, and gene expression programs involved in cellular fate determination. Positive immunoreactivity confirms detection of endogenous IKZF1 and supports the utility of this antibody for immunofluorescence applications. Nuclei are counterstained with DAPI (blue). Enzymatic antigen retrieval was performed for 15 min prior to staining, followed by incubation with IKZF1 antibody (5 ug/ml) overnight at 4°C and DyLight 488-conjugated secondary antibody detection.

IKZF1 Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue using IKZF1 Antibody / Lymphoid Development Transcription Factor Antibody demonstrates prominent nuclear HRP-DAB brown staining within subsets of lymphoid cells. The staining pattern is consistent with expression of IKZF1 (Ikaros), a master regulator of lymphoid lineage commitment that controls gene expression programs required for lymphocyte differentiation, immune cell maturation, and hematopoietic development. Strong nuclear localization is expected for this DNA-binding transcription factor and highlights its central role in regulating the formation and maintenance of lymphoid cell populations. These findings support the utility of this antibody for immunohistochemical detection of endogenous IKZF1 in lymphoid tissues. HIER: boil paraffin sections in pH 6, 10mM citrate buffer for 20 min and allow to cool prior to staining.

IKZF1 Antibody Mouse Spleen IHC. Immunohistochemical staining of FFPE mouse spleen tissue using IKZF1 Antibody / Lymphoid Development Transcription Factor Antibody demonstrates distinct nuclear HRP-DAB brown staining within scattered lymphoid cell populations. The staining pattern is consistent with expression of IKZF1 (Ikaros), a master regulator of lymphoid development that controls transcriptional programs required for lymphocyte differentiation, immune cell maturation, and hematopoietic lineage commitment. Predominantly nuclear immunoreactivity is expected for this DNA-binding transcription factor and reflects its central role in regulating gene expression during immune system development and maintenance. These findings support the utility of this antibody for immunohistochemical detection of endogenous IKZF1 in lymphoid tissues. HIER: boil paraffin sections in pH 6, 10mM citrate buffer for 20 min and allow to cool prior to staining.

IKZF1 Antibody Rat Spleen IHC. Immunohistochemical staining of FFPE rat spleen tissue using IKZF1 Antibody / Lymphoid Development Transcription Factor Antibody demonstrates distinct nuclear HRP-DAB brown staining within lymphoid cell populations. The staining pattern is consistent with expression of IKZF1 (Ikaros), a master regulator of lymphoid lineage commitment that directs transcriptional programs required for lymphocyte differentiation, immune cell maturation, and hematopoietic development. Positive nuclear immunoreactivity is expected for this DNA-binding transcription factor and highlights its essential role in regulating gene expression networks that govern normal lymphoid cell function and immune system formation. The observed staining supports the utility of this antibody for immunohistochemical detection of endogenous IKZF1 in lymphoid tissues. HIER: boil paraffin sections in pH 6, 10mM citrate buffer for 20 min and allow to cool prior to staining.

IKZF1 Antibody Daudi WB. Western blot analysis of human Daudi cell lysate using IKZF1 Antibody / Ikaros Family Zinc Finger 1 Antibody demonstrates multiple immunoreactive bands between approximately 50–70 kDa, consistent with the known molecular weight range of IKZF1 (Ikaros). IKZF1 undergoes extensive alternative splicing, generating multiple protein isoforms that differ in DNA-binding and regulatory domains, resulting in the characteristic multi-band pattern frequently observed in lymphoid cells. As a master regulator of hematopoietic and lymphoid differentiation, IKZF1 plays essential roles in immune cell development, transcriptional regulation, and chromatin remodeling. Detection of multiple endogenous IKZF1 isoforms in Daudi cells supports the specificity of this antibody for western blot applications. Predicted molecular weight: ~50–70 kDa. Observed molecular weights: ~50–70 kDa.

IKZF1 Antibody U937 Flow Cytometry. Flow cytometric analysis of human U937 cells stained with IKZF1 Antibody / Ikaros Family Zinc Finger 1 Antibody demonstrates a pronounced rightward shift of the antibody-stained population (blue) relative to both the isotype control (green) and unstained cells (red). The observed fluorescence profile is consistent with expression of IKZF1 (Ikaros), a zinc finger transcription factor that functions as a master regulator of hematopoietic differentiation, lymphoid development, and transcriptional control of immune-related gene expression. The clear separation between the IKZF1-positive and control populations supports specific detection of endogenous IKZF1 by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized, and stained with IKZF1 antibody (1 ug/10^6 cells), followed by DyLight 488-conjugated secondary antibody detection. Red = unstained cells, Green = isotype control, Blue = IKZF1 antibody.