IHH Antibody / Indian hedgehog protein (FY13441)

Immunofluorescence analysis of Indian hedgehog protein using IHH antibody. Indian hedgehog protein expression was examined in a paraffin-embedded section of human liver cancer tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with IHH antibody overnight at 4°C. Detection was performed using a Cy3-conjugated secondary antibody, and nuclei were counterstained with DAPI. Fluorescent images were acquired using appropriate filter sets.

Immunohistochemistry analysis of Indian hedgehog protein using IHH antibody. Indian hedgehog protein expression was examined in a paraffin-embedded section of human liver cancer tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with IHH antibody overnight at 4°C. Signal was detected using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.

Immunohistochemistry analysis of Indian hedgehog protein using IHH antibody. Indian hedgehog protein expression was examined in a paraffin-embedded section of human kidney tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with IHH antibody overnight at 4°C. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.

Western blot analysis of Indian hedgehog protein using IHH antibody. Lane 1: human Caco-2 whole cell lysates; Lane 2: human HepG2 whole cell lysates; Lane 3: human SiHa whole cell lysates; Lane 4: rat kidney tissue lysates; Lane 5: mouse small intestine tissue lysates; Lane 6: mouse kidney tissue lysates. A primary band is detected at approximately 45 kDa, consistent with the predicted molecular weight of full-length Indian hedgehog protein. Additional weaker bands observed between 45-50 kDa and at approximately 70 kDa may represent alternative processing states, post-translationally modified forms, or higher-order species commonly reported for hedgehog family proteins. The overall banding pattern is consistent with detection of IHH in human and rodent samples.

Flow cytometry analysis of fixed human RT4 cells with IHH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IHH antibody.