IGFBP3 Antibody IGFBP3/3424 / BP-53 Antibody [clone IGFBP3/3424] (V9555)

IGFBP3 Antibody Staining in U-87 MG Glioblastoma Cells IF. Immunofluorescence staining of PFA-fixed human U-87 MG glioblastoma cells with IGFBP3 antibody clone IGFBP3/3424 shows strong green fluorescence with predominantly cytoplasmic staining, including prominent perinuclear signal. Phalloidin counterstaining (red) highlights the actin cytoskeleton and cellular morphology. The image demonstrates clear IGFBP3 immunoreactivity throughout the cytoplasmic compartment.

IGFBP3 Antibody in U-87 MG Glioblastoma Cells FACS. Flow cytometry analysis of PFA-fixed human U-87 MG glioblastoma cells using IGFBP3 antibody clone IGFBP3/3424 demonstrates a strong rightward fluorescence shift in the antibody-stained population (blue) compared with the isotype control (red). The well-separated populations indicate robust detection of intracellular IGFBP3 under the tested conditions.

Protein Microarray Validated IGFBP3 Antibody Specificity. HuProt protein microarray analysis of IGFBP3 antibody clone IGFBP3/3424 against more than 19,000 full-length human proteins demonstrates preferential recognition of IGFBP3. The intended target ranks first with a strong signal and clear separation from the next-ranked proteins. The Z-score represents antibody signal strength in standard deviations above the mean signal across the array, while the S-score represents the difference between successive ranked Z-scores and therefore reflects relative target specificity. This Protein Microarray Validated result supports selective recognition of IGFBP3 by clone IGFBP3/3424.