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Home >> Antibodies >> IGF2-AS Antibody

IGF2-AS Antibody (RQ6136)

  Catalog No Formulation Size Price (USD)  
Image RQ6136 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
Immunofluorescent staining of FFPE human U-2 OS cells with IGF2-AS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human PANC-1 cell lysate with IGF2-AS antibody. Predicted molecular weight ~18 kDa.
Flow cytometry testing of human Caco-2 cells with IGF2-AS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IGF2-AS antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q6U949
Applications Western blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This IGF2-AS antibody is available for research use only.
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Description

This gene is expressed in antisense to the insulin-like growth factor 2 (IGF2) gene and is imprinted and paternally expressed. It is thought to be non-coding because the putative protein is not conserved and translation is predicted to trigger nonsense mediated decay (NMD). Transcripts from this gene are produced in tumors and may function to suppress cell growth. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the IGF2-AS antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids M1-K168) was used as the immunogen for the IGF2-AS antibody.

Storage

After reconstitution, the IGF2-AS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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