IGF1R Antibody IGF1R/4667 / Insulin-Like Growth Factor 1 Receptor Antibody [clone IGF1R/4667] (V8864)

IGF1R Antibody Staining in Human Prostate Carcinoma IHC. Immunohistochemistry of FFPE human prostate carcinoma tissue using IGF1R antibody clone IGF1R/4667 shows strong brown staining in the epithelial tumor cells, with prominent membranous and cytoplasmic signal. Surrounding stromal tissue shows comparatively little staining, providing good contrast with the positively stained carcinoma. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing.

IGF1R Antibody Staining in Human Breast Carcinoma IHC. Immunohistochemistry of FFPE human breast carcinoma tissue using IGF1R antibody clone IGF1R/4667 shows strong brown staining of glandular carcinoma structures. Signal is prominent along the cell membranes with accompanying cytoplasmic staining, while the surrounding stromal tissue exhibits substantially weaker reactivity. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing.

IGF1R Antibody Staining in Human Colon Carcinoma IHC. Immunohistochemistry of FFPE human colon carcinoma tissue using IGF1R antibody clone IGF1R/4667 demonstrates brown staining within tumor cell clusters, with predominantly cytoplasmic and membranous reactivity. The surrounding stromal compartment shows comparatively limited staining, helping delineate the positive carcinoma cells. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing.

Purified IGF1R/4667 Monoclonal Antibody SDS-PAGE. SDS-PAGE analysis of purified, BSA-free IGF1R antibody clone IGF1R/4667 under non-reducing (NR) and reducing (R) conditions. A predominant band is visible at approximately 140-150 kDa under non-reducing conditions, consistent with intact mouse IgG, while reduction produces a prominent band near 25 kDa corresponding to the immunoglobulin light chain; the heavy-chain region is also visible near 50 kDa. Two micrograms of purified antibody were loaded per lane. The profile supports the integrity and purity of the monoclonal antibody preparation.

Protein Microarray Validated IGF1R Antibody Specificity. HuProt protein microarray analysis of IGF1R antibody clone IGF1R/4667 against more than 19,000 full-length human proteins demonstrates strong preferential recognition of IGF1R. The intended target ranks first with a Z-score of approximately 126.75 and an S-score of approximately 115.53, markedly separated from the next-ranked proteins. The Z-score represents signal strength in standard deviations above the mean signal across the array, while the S-score represents the difference between successive ranked Z-scores and therefore indicates relative target specificity. This Protein Microarray Validated result demonstrates highly selective recognition of IGF1R by clone IGF1R/4667.