IgD Heavy Chain Antibody for IHC | IGHD | MSVA-701R

	Catalog No	Formulation	Size	Price (USD)
	V6088-100UG	Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide	100 ug	654.50
Formulation
	V6088-20UG	Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide	20 ug	324.50
Formulation

IgD Heavy Chain Antibody for IHC immunohistochemistry analysis of IgD in human tissue microarrays. IgD Heavy Chain Antibody for IHC (clone MSVA-701R) was evaluated on formalin-fixed paraffin-embedded human tissue microarrays containing a broad range of normal and cancer tissues. HRP-DAB chromogenic staining highlights IgD-positive lymphocytes within lymphoid tissues, particularly in mantle zone B cells of tonsil, lymph node, and spleen, while most non-lymphoid tissues show minimal staining. In cancer tissue arrays, staining is primarily observed in infiltrating B lymphocytes within tumor microenvironments rather than tumor epithelial cells. The observed immunohistochemistry patterns are consistent with known IgD / IGHD expression profiles reported in the Human Protein Atlas.

Species Reactivity	Human
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	MSVA-701R
UniProt	P01880
Localization	Cytoplasm
Applications	Immunohistochemistry (FFPE) : 1:100-1:200
Limitations	This IgD Heavy Chain/IGHD antibody is available for research use only.
	Review this product on BioCompare and get a $20 Amazon gift card

Description

Immunoglobulin D (IgD) is an antibody isotype expressed primarily on the surface of mature naive B lymphocytes and plays an important role in B cell activation and immune regulation. The IgD heavy chain is encoded by the IGHD gene and forms part of the B cell receptor complex together with immunoglobulin light chains. IgD Heavy Chain Antibody for IHC is used to detect this immunoglobulin component in tissue sections, enabling visualization of IgD-expressing B cell populations within lymphoid organs and immune infiltrates.

IgD belongs to the immunoglobulin superfamily and is structurally composed of two delta heavy chains paired with two light chains, forming a monomeric antibody molecule. Membrane-bound IgD functions as an antigen receptor on mature B cells where it cooperates with surface IgM to regulate B cell activation, antigen recognition, and signaling. Engagement of the B cell receptor containing IgD can initiate intracellular signaling cascades that influence lymphocyte survival, differentiation, and antibody production.

In normal human tissues, IgD expression is most prominently detected in B lymphocytes within secondary lymphoid organs such as tonsil, lymph node, and spleen. Within lymphoid follicles, IgD positive B cells are typically enriched in mantle zone regions surrounding germinal centers. This characteristic distribution makes IgD immunohistochemical staining a useful tool for evaluating lymphoid tissue architecture and identifying specific B cell subsets within reactive or neoplastic lymphoid proliferations.

IgD heavy chain antibody detection can also provide diagnostic information in hematopathology. Immunohistochemical staining for IgD may help characterize B cell lineage and maturation stage in certain lymphoid malignancies or plasma cell disorders. In research contexts, detection of IgD contributes to studies of B cell biology, antigen receptor signaling, and the organization of immune cell populations within lymphoid tissues.

A recombinant rabbit monoclonal IgD Heavy Chain Antibody for IHC such as clone MSVA-701R enables sensitive detection of IgD expressing cells in formalin-fixed paraffin-embedded tissue sections. Immunohistochemistry staining typically demonstrates membranous and cytoplasmic localization in B lymphocytes consistent with the distribution of IgD as part of the B cell receptor complex.

Application Notes

1. Optimal dilution of the IgD Heavy Chain Antibody for IHC (clone MSVA-701R) should be determined by the researcher.

2. This IGHD/Immunoglobulin heavy constant delta antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

A recombinant fragment (around amino acids 1-200) of human IGHD protein (exact sequence is proprietary) was used as the immunogen for the IgD Heavy Chain Antibody for IHC.