- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
IgD Antibody for WB / Immunoglobulin D Western Blot Antibody [clone AFBO-9] (RQ5075)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5075 | Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA | 100 ul | 449 |
|
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | AFBO-9 |
| Purity | Affinity purified |
| UniProt | P01880 |
| Applications | Western Blot : 1:500-1:2000 |
| Limitations | This IgD antibody is available for research use only. |
|
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Immunoglobulin D (IgD) is one of the five major antibody isotypes produced by B lymphocytes and is encoded by the IGHD gene. IgD functions primarily as a membrane-bound component of the B cell receptor complex on mature naive B lymphocytes where it participates in antigen recognition and immune activation. IgD Antibody for WB is designed for detection of Immunoglobulin D in protein samples separated by SDS-PAGE and analyzed by western blotting.
IgD belongs to the immunoglobulin superfamily and is composed of two delta heavy chains paired with two immunoglobulin light chains. In serum and plasma, IgD is typically present as a monomeric immunoglobulin molecule. During western blot analysis under reducing conditions, disulfide bonds linking the heavy and light chains are disrupted, allowing the IgD heavy chain to migrate independently during electrophoresis. The IgD delta heavy chain typically resolves near approximately 55-60 kDa on SDS-PAGE gels, corresponding to the molecular mass of the IGHD gene product.
Western blot analysis of immunoglobulins is commonly used to evaluate antibody composition, confirm immunoglobulin isotype identity, and detect immunoglobulin heavy chains in biological samples such as serum, plasma, or purified antibody preparations. Detection of the IgD heavy chain is particularly useful for distinguishing IgD from other immunoglobulin classes such as IgG, IgA, IgM, and IgE, each of which contains structurally distinct heavy chain proteins that migrate at characteristic molecular weights.
In immunology and antibody engineering studies, western blot detection of IgD can support analysis of B cell receptor expression, immunoglobulin secretion, and antibody purification workflows. Because IgD is present in relatively low concentrations in human serum compared with IgG or IgA, sensitive antibody reagents are required for reliable detection following electrophoretic separation and membrane transfer.
A recombinant rabbit monoclonal IgD Antibody for WB such as clone AFBO-9 provides a highly specific reagent for detecting the IgD heavy chain in western blot experiments. This antibody can be used to identify IgD in serum samples, purified immunoglobulin preparations, or B cell-derived protein lysates, enabling researchers to analyze immunoglobulin expression and confirm the presence of the IGHD heavy chain in biochemical assays.
Optimal dilution of the IgD Antibody for WB should be determined by the researcher.
A synthetic peptide specific to human IGHD was used as the immunogen for the IgD antibody.
Store the IgD antibody at -20oC.
Immunoglobulin D antibody, IgD antibody, IGHD antibody, IgD heavy chain antibody
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