• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> IFNG Antibody / Interferon gamma

IFNG Antibody / Interferon gamma (RQ4575)

  Catalog No Formulation Size Price (USD)  
Image RQ4575 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
Bulk quote request
IFNG Antibody Staining of Human Tonsil IHC. Immunohistochemistry of FFPE human tonsil tissue using IFNG Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 min followed by cooling before staining. The image shows widespread brown immunoreactivity among cells within the tonsillar tissue, with areas of stronger staining and comparatively less reactive lymphoid regions. This staining pattern demonstrates detection by the Interferon gamma Antibody in the tested human tonsil section.
IFNG Antibody Staining of Mouse Colon IHC. Immunohistochemistry of FFPE mouse colon tissue using IFNG Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 min followed by cooling before staining. The image shows brown immunoreactivity predominantly along the basolateral and interglandular regions of the colonic epithelium, while the epithelial nuclei and much of the cytoplasm remain comparatively unstained. This pattern demonstrates detection by the Interferon gamma Antibody in the tested mouse colon section.
IFNG Antibody Detection in Human, Rat, and Mouse Tissues WB. Western blot analysis of human placenta (lane 1), rat spleen (lane 2), mouse spleen (lane 3), and mouse thymus (lane 4) tissue lysates using IFNG Antibody at 0.5 ug/ml. A discrete band is detected at approximately 20-22 kDa in all four samples, consistent with the expected molecular weight range of 19-24 kDa for interferon gamma depending on glycosylation. The results demonstrate detection of Interferon gamma across the tested human, rat, and mouse tissue lysates.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P01579
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
ELISA (Capture) : 1-5ug/ml (human recombinant protein)
Limitations This IFNG antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

IFNG antibody targets Interferon gamma, a soluble cytokine encoded by the IFNG gene that plays a central role in innate and adaptive immune responses. Interferon gamma is primarily produced by activated T lymphocytes, natural killer cells, and certain innate lymphoid cell populations. It is secreted into the extracellular space, where it binds to its cognate receptor on target cells to initiate downstream signaling events that shape immune activation, host defense, and immune regulation.

Functionally, Interferon gamma is a key immunomodulatory cytokine that promotes macrophage activation, enhances antigen presentation, and drives T helper 1 type immune responses. A short functional summary is that IFNG coordinates cellular immunity by linking lymphocyte activation to antimicrobial and inflammatory effector functions. Through induction of interferon stimulated genes, Interferon gamma enhances expression of major histocompatibility complex molecules and supports efficient recognition of infected or transformed cells.

At the molecular level, Interferon gamma is synthesized as a monomer that forms a biologically active homodimer upon secretion. Binding of IFNG to the interferon gamma receptor activates the JAK STAT signaling pathway, leading to transcriptional regulation of genes involved in immune defense, inflammation, and cell cycle control. IFNG antibody reagents are widely used to study cytokine production, secretion dynamics, and immune cell activation in both in vitro and in vivo research settings.

From a biological and disease relevance perspective, IFNG plays a critical role in host defense against intracellular pathogens, including viruses, bacteria, and parasites. Dysregulated Interferon gamma signaling has been implicated in autoimmune disease, chronic inflammation, immunodeficiency, and cancer. Elevated or sustained IFNG expression can contribute to tissue damage in inflammatory conditions, while insufficient IFNG activity is associated with impaired immune responses. IFNG antibody tools are therefore essential for research in immunology, infectious disease, and immune mediated pathology.

Developmentally, IFNG expression is tightly controlled and inducible, reflecting its potent biological activity. Its production is closely linked to immune cell differentiation and activation state. IFNG antibodies from NSJ Bioreagents are supplied for research use to support studies of cytokine signaling, immune regulation, and cellular immune responses.

For an alternative recombinant monoclonal clone targeting IFNG, see our Interferon gamma Antibody rIFNG/4467 / Type II Interferon Antibody page.

Application Notes

Optimal dilution of the IFNG antibody should be determined by the researcher.

Immunogen

Amino acids Q24-G161 from the human protein were used as the immunogen for the IFNG antibody.

Storage

After reconstitution, the IFNG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.