IFITM3 Antibody | F54274 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	F54274-0.4ML	In 1X PBS, pH 7.4, with 0.09% sodium azide	0.4 ml	399
Formulation
	F54274-0.08ML	In 1X PBS, pH 7.4, with 0.09% sodium azide	0.08 ml	185
Formulation

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Western blot testing of human 1) HeLa, 2) 293, 3) A549, 4) NCI-H460, 5) HepG2, 6) SK-B-R3 and 7) A375 cell lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).

Western blot testing of human 1) HeLa, 2) 293, 3) HepG2, 4) NCI-H460, 5) SK-B-R3, 6) placent and 7) mouse colon lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).

IHC testing of FFPE human spleen tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

IHC testing of FFPE human colon carcinoma tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Immunofluorescent staining of fixed and permeabilized human HeLa cells with IFITM3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Availability	1-3 business days
Species Reactivity	Human, Mouse
Format	Purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit Ig
Purity	Antigen affinity purified
UniProt	Q01628
Localization	Cytoplasmic
Applications	Western blot : 1:500-1:2000 Immunohistochemistry (FFPE) : 1:25 Immunofluorescence : 1:25
Limitations	This IFITM3 antibody is available for research use only.
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Description

The family of interferon-induced transmembrane protein (Ifitm/mil/fragilis) cell surface proteins may modulate cell adhesion and influence cell differentiation.

Application Notes

The stated application concentrations are suggested starting points. Titration of the IFITM3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.