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Home >> Antibodies >> IDO2 Antibody / INDOL1

IDO2 Antibody / INDOL1 [clone IDO2/2640] (V9631)

  Catalog No Formulation Size Price (USD)  
Image V9631-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9631-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9631SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IDO2 antibody (clone IDO2/2640). These results demonstrate the foremost specificity of the IDO2/2640 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human placental tissue with IDO2 antibody (clone IDO2/2640) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG, kappa
Clone Name IDO2/2640
Purity Protein A/G affinity
UniProt Q6ZQW0
Localization Cytoplasm
Applications ELISA (order BSA-free format for coating) :
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This IDO2 antibody is available for research use only.
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Description

IDO2 is a presumptive immunomodulatory gene based on its close structural relationship to IDO1 and its expression in a variety of antigen-presenting cell types. Both IDO1 and IDO2 will catabolize tryptophan to kynurenine. Biochemical studies indicate that both enzymes are similarly robust in catabolic activity, although the in vitro conditions required for IDO2 to manifest the same level of activity differ somewhat from IDO1. However, whether IDO2 is active as a tryptophan catabolizing enzyme in human dendritic cells has been disputed. Further work is needed to conclusively determine that IDO1 and IDO2 are similar in their preference for substrates and reaction pathways to generate product. A non-redundant function for IDO2 relative to IDO1 is suggested by their rather distinct expression patterns and response to extracellular stimuli.

Application Notes

Optimal dilution of the IDO2 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 200-350 was used as the immunogen for the IDO2 antibody.

Storage

Aliquot the IDO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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