IDH1 Antibody IDH1/1152 / Cytosolic Isocitrate Dehydrogenase Antibody [clone IDH1/1152] (V2609)

Description

IDH1 Antibody IDH1/1152 / Cytosolic Isocitrate Dehydrogenase Antibody recognizes isocitrate dehydrogenase 1 (IDH1), a cytosolic and peroxisomal enzyme involved in cellular metabolism and redox regulation. IDH1 catalyzes the NADP-dependent oxidative decarboxylation of isocitrate to alpha-ketoglutarate, generating NADPH in the process. Through production of NADPH and alpha-ketoglutarate, IDH1 contributes to lipid biosynthesis, maintenance of cellular redox balance, and metabolic processes that support normal cell function.

IDH1 is distinct from the mitochondrial isocitrate dehydrogenases and functions primarily outside the mitochondrial tricarboxylic acid cycle. Cytosolic isocitrate dehydrogenase activity provides a source of NADPH used in reductive biosynthetic reactions and antioxidant defense. IDH1 therefore links intermediary metabolism with cellular responses to oxidative stress and changing metabolic demands. Alterations in IDH1 expression or activity can influence metabolic homeostasis, redox state, and the availability of metabolites used by other cellular pathways.

IDH1 is also an important target in cancer biology because recurrent somatic mutations occur at arginine 132. Mutant IDH1 proteins acquire neomorphic enzymatic activity that converts alpha-ketoglutarate to D-2-hydroxyglutarate, an oncometabolite capable of altering alpha-ketoglutarate-dependent cellular processes. IDH1 mutations are particularly associated with subsets of glioma, acute myeloid leukemia, chondrosarcoma, and other malignancies. Antibodies recognizing general IDH1 protein complement mutation-specific reagents by enabling investigation of IDH1 expression independently of a particular R132 substitution.

Clone IDH1/1152 is a mouse monoclonal antibody generated against a recombinant fragment corresponding to approximately amino acids 280-420 of the human protein. NSJ Bioreagents offers this Cytosolic Isocitrate Dehydrogenase Antibody for researchers investigating IDH1 expression, cellular metabolism, redox biology, and cancer-associated metabolic alterations. An IDH1 Antibody provides a clone-defined reagent for studying this metabolically important enzyme and its relationship to normal and disease-associated cellular processes.

Explore our Metabolism Antibodies page for additional reagents targeting enzymes and regulatory proteins involved in cellular metabolism, energy homeostasis, and metabolic signaling.

Application Notes

Optimal dilution of the IDH1 Antibody IDH1/1152 / Cytosolic Isocitrate Dehydrogenase Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 or 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 Antibody IDH1/1152.

Storage

Store the IDH1 Antibody IDH1/1152 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

IDH1 antibody, Isocitrate dehydrogenase 1 antibody, Cytosolic isocitrate dehydrogenase antibody, Isocitrate dehydrogenase [NADP] cytoplasmic antibody, IDH antibody