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Home >> Antibodies >> IDH1 Antibody

IDH1 Antibody (F50422)

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  Catalog No Formulation Size Price (USD)  
Image F50422-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F50422-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from (1) HepG2, (2) MCF-7 cell line, (3) human liver and (4) rat liver tissue using IDH1 antibody at 1:1000. Predicted molecular weight ~46 kDa.
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Western blot analysis of lysate from (1) HepG2, (2) MCF-7 cell line, (3) human liver and (4) rat liver tissue using IDH1 antibody at 1:1000. Predicted molecular weight ~46 kDa.
Western blot analysis of IDH1 antibody and HepG2 cell line and mouse liver tissue lysate. Predicted molecular weight ~46 kDa.
Confocal immunofluorescent analysis of IDH1 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human prostate section using IDH1 antibody; Ab was diluted at 1:100.
IHC analysis of FFPE human hepatocarcinoma tissue stained with IDH1 antibody
IDH1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt O75874
Localization Cytoplasmic, nuclear
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This IDH1 antibody is available for research use only.
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Description

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [Wiki]

Application Notes

Titration of the IDH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 116-143 from the human protein was used as the immunogen for this IDH1 antibody.

Storage

Aliquot the IDH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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