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Home >> Antibodies >> IDH Antibody

IDH Antibody (F50421)

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  Catalog No Formulation Size Price (USD)  
Image F50421-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50421-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Confocal immunofluorescent analysis of IDH antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI nuclear counterstain (blue).
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Confocal immunofluorescent analysis of IDH antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI nuclear counterstain (blue).
IHC analysis of FFPE human brain stained with IDH antibody
Western blot analysis of IDH antibody and mouse liver tissue and 293 lysate. Predicted molecular weight ~46 kDa.
Flow cytometric analysis of 293 cells using IDH antibody (bottom histogram) compared to a negative control (top histogram).
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt O75874
Localization Cytoplasmic, nuclear
Applications Immunofluorescence : 1:10-1:50
Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This IDH antibody is available for research use only.
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Description

The protein is the NADP(+)-dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions. [Wiki]

Application Notes

Titration of the IDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 63-90 from the human protein was used as the immunogen for this IDH antibody.

Storage

Aliquot the IDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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