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Home >> Antibodies >> ID1 Antibody

ID1 Antibody [clone PCRP-ID1-2F11] (V8940)

  Catalog No Formulation Size Price (USD)  
Image V8940-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8940-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8940SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Immunofluorescent staining of PFA-fixed human HeLa cells using ID1 antibody (green, clone PCRP-ID1-2F11) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ID1 antibody (blue, clone PCRP-ID1-2F11) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free ID1 antibody (clone PCRP-ID1-2F11) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ID1 antibody (clone PCRP-ID1-2F11). These results demonstrate the foremost specificity of the PCRP-ID1-2F11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name PCRP-ID1-2F11
Purity Protein A/G affinity
UniProt P41134
Localization Nucleus, Cytoplasm
Applications ELISA (order BSA-free format for coating) :
Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This ID1 antibody is available for research use only.
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  • Applications : WB, IHC, IF, FACS, ELISA
    Reactivity : Human, Mouse
    Pred. Reactivity : Rat

Description

Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression.

Application Notes

Optimal dilution of the ID1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 5-150 was used as the immunogen for the ID1 antibody.

Storage

Aliquot the ID1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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