ICOSL Antibody / T-Cell Costimulation Marker [clone ICOSL/3111] (V7578)

ICOSL Antibody U937 Cell IF. Immunofluorescence staining of human U937 cells using clone ICOSL/3111 (green) and Reddot nuclear counterstain (red). Inducible T-cell costimulator ligand / ICOSLG displays predominantly membranous and cytoplasmic fluorescence consistent with its role as a B7 family immune checkpoint ligand involved in antigen-presenting cell communication and adaptive T-cell costimulation signaling pathways.

ICOSL Antibody U937 Cell FACS. Flow cytometry analysis of human U937 cells stained with clone ICOSL/3111 (blue) and matched isotype control (red). The observed fluorescence shift demonstrates surface-associated expression of Inducible T-cell costimulator ligand / ICOSLG, consistent with its role as a B7 family costimulatory ligand involved in antigen-presenting cell signaling and adaptive immune activation pathways regulating T-cell responses.

ICOSL Antibody Prostate Carcinoma IHC. Immunohistochemistry analysis of FFPE human prostate carcinoma tissue stained with clone ICOSL/3111. Tumor epithelial cells display strong membranous and cytoplasmic HRP-DAB brown staining consistent with expression of Inducible T-cell costimulator ligand / ICOSLG, a B7 family immune regulatory protein involved in adaptive immune signaling and T-cell costimulation pathways within the tumor microenvironment. Adjacent stromal regions show comparatively lower staining intensity. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

ICOSL Antibody Colon Carcinoma IHC. Immunohistochemistry analysis of FFPE human colon carcinoma tissue stained with clone ICOSL/3111. Tumor epithelial cells demonstrate membranous and cytoplasmic HRP-DAB brown staining consistent with expression of Inducible T-cell costimulator ligand / ICOSLG, an immune checkpoint-associated B7 family ligand involved in adaptive immune regulation and T-cell costimulation signaling within the tumor microenvironment. Stromal connective tissue shows comparatively reduced staining intensity. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

ICOSL Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone ICOSL/3111. These results demonstrate high specificity of the mouse monoclonal antibody for Inducible T-cell costimulator ligand / ICOSLG, a B7 family immune checkpoint ligand involved in adaptive immune activation and T-cell costimulation signaling. Z- and S-score analysis confirms strong preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.

SDS-PAGE analysis of purified, BSA-free ICOSLG / ICOSL antibody (clone ICOSL/3111) as confirmation of integrity and purity.