ICOS Ligand Antibody / Immune Checkpoint Ligand [clone ICOSL/3260] (V7774)

ICOS Ligand Antibody Colon Carcinoma IHC. Immunohistochemistry analysis of FFPE human colon carcinoma tissue stained with clone ICOSL/3260. Tumor epithelial cells display diffuse membranous and cytoplasmic HRP-DAB brown staining consistent with expression of Inducible T-cell costimulator ligand / ICOSLG, an immune checkpoint-associated B7 family ligand involved in adaptive immune signaling and regulation of T-cell activation within the tumor microenvironment. Stromal elements demonstrate comparatively weaker background staining. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

ICOS Ligand Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone ICOSL/3260. These results demonstrate high specificity of the mouse monoclonal antibody for Inducible T-cell costimulator ligand / ICOSLG, an immune checkpoint-associated B7 family protein involved in adaptive immune signaling and T-cell activation pathways. Z- and S-score analysis confirms strong preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.

SDS-PAGE analysis of purified, BSA-free ICOS Ligand antibody (clone ICOSL/3260) as confirmation of integrity and purity.