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Home >> Antibodies >> ICOS Antibody

ICOS Antibody (RQ4012)

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  Catalog No Formulation Size Price (USD)  
Image RQ4012 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) mouse stomach, 2) mouse spleen, 3) mouse thymus, 4) mouse small intestine, 5) rat thymus and 6) human placental lysate with ICOS antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa.
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Western blot testing of 1) mouse stomach, 2) mouse spleen, 3) mouse thymus, 4) mouse small intestine, 5) rat thymus and 6) human placental lysate with ICOS antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa.
IHC testing of FFPE mouse spleen tissue with ICOS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse thymus tissue with ICOS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with ICOS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9WVS0
Localization Cytoplasmic, cell membrane
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This ICOS antibody is available for research use only.
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Description

Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Application Notes

Optimal dilution of the ICOS antibody should be determined by the researcher.

Immunogen

A recombinant mouse partial protein corresponding to amino acids E21-S200 was used as the immunogen for the ICOS antibody.

Storage

After reconstitution, the ICOS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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