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Home >> Antibodies >> ICAM1 Antibody

ICAM1 Antibody (RQ7001)

  Catalog No Formulation Size Price (USD)  
Image RQ7001 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HepG2 cells with ICAM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM1 antibody.
Western blot testing of human 1) Ramos and 2) Raji cell lysate with ICAM1 antibody. Predicted molecular weight: ~58 kDa (unmodified), 75-115 kDa (glycosylated).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P05362
Applications Western blot : 0.5-1 ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ICAM1 antibody is available for research use only.
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Description

CD54, also known as ICAM-1 and Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. This protein is the major human rhinovirus receptor. The ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).

Application Notes

Optimal dilution of the ICAM1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids D53-K519) was used as the immunogen for the ICAM1 antibody.

Storage

After reconstitution, the ICAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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