AIF1 Antibody / Microglia Detection Antibody (R32736)

AIF1 Antibody Mouse Brain IHC. Immunohistochemistry analysis of paraffin-embedded mouse brain tissue using AIF1 Antibody demonstrates HRP-DAB brown cytoplasmic staining in microglial cells distributed throughout the neural parenchyma, with characteristic ramified morphology and fine cellular processes consistent with AIF1 / IBA1 expression as a microglia marker involved in neuroinflammatory response and immune surveillance, while neurons and glial cells remain largely negative; nuclei are counterstained blue. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

AIF1 Antibody Mouse Brain IHC. Immunohistochemistry analysis of paraffin-embedded mouse brain tissue using AIF1 Antibody demonstrates HRP-DAB brown cytoplasmic staining in scattered microglial cells within the neural parenchyma, showing characteristic ramified morphology with fine branching processes consistent with AIF1 / IBA1 expression as a microglia marker involved in neuroimmune surveillance and inflammatory activation, while surrounding neurons and other glial cells remain largely negative; nuclei are counterstained blue. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

AIF1 Antibody Rat Brain IHC. Immunohistochemistry analysis of paraffin-embedded rat brain tissue using AIF1 Antibody demonstrates HRP-DAB brown cytoplasmic staining in microglial cells distributed throughout the neural parenchyma, including regions adjacent to neuronal layers, with characteristic ramified morphology and fine processes consistent with AIF1 / IBA1 expression as a microglia marker involved in neuroinflammatory surveillance, while neurons remain largely negative; nuclei are counterstained blue. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

AIF1 Antibody Rat Brain IHC. Immunohistochemistry analysis of paraffin-embedded rat brain tissue using AIF1 Antibody demonstrates HRP-DAB brown cytoplasmic staining in dispersed microglial cells throughout the neural parenchyma, exhibiting characteristic ramified morphology with elongated processes consistent with AIF1 / IBA1 expression as a microglia marker associated with neuroimmune surveillance and activation, while surrounding neuronal cells remain largely negative; nuclei are counterstained blue. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

AIF1 Antibody Pig Brain IHC. Immunohistochemistry analysis of paraffin-embedded pig brain tissue using AIF1 Antibody demonstrates HRP-DAB brown cytoplasmic staining in scattered microglial cells throughout the neural parenchyma, displaying characteristic ramified morphology with fine branching processes consistent with AIF1 / IBA1 expression as a microglia marker involved in neuroimmune surveillance and inflammatory response, while surrounding neuronal cells remain largely negative; nuclei are counterstained blue. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

AIF1 Antibody Spleen IHC. IBA1/AIF-1 was detected in paraffin-embedded sections of human spleen tissue. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0). The tissue sections were blocked with 10% goat serum and incubated with rabbit anti-IBA1/AIF-1 antibody overnight at 4°C. Peroxidase-conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue sections were developed using an HRP-conjugated detection system with DAB as the chromogen.

AIF1 Antibody Human Spleen Tissue IHC. IBA1/AIF-1 was detected in paraffin-embedded sections of human spleen tissue. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0). The tissue sections were blocked with 10% goat serum and incubated with rabbit anti-IBA1/AIF-1 antibody overnight at 4°C. Peroxidase-conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue sections were developed using an HRP-conjugated detection system with DAB as the chromogen.

AIF1 Antibody WB. IBA1/AIF-1 antibody immunoreactivity is observed in human HEL and THP-1 whole cell lysate western blot. Proteins were separated by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. Lane 1: human HEL whole cell lysates; Lane 2: human THP-1 whole cell lysates. A distinct band is detected at approximately 17 kDa, consistent with the predicted molecular weight of IBA1/AIF-1.