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Home >> Antibodies >> IARS1 Antibody / Isoleucine--tRNA ligase, cytoplasmic

IARS1 Antibody / Isoleucine--tRNA ligase, cytoplasmic (RQ8507)

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  Catalog No Formulation Size Price (USD)  
Image RQ8507 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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IHC staining of FFPE human liver cancer tissue with IARS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer tissue with IARS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with IARS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with IARS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562 and 2) HeLa cell lysate with IARS1 antibody. Predicted molecular weight ~144 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with IARS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IARS1 antibody.
Availability 1-3 days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P41252
Localization Cytoplasm
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This IARS1 antibody is available for research use only.
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Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found.

Application Notes

Optimal dilution of the IARS1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids E812-E1056) was used as the immunogen for the IARS1 antibody.

Storage

After reconstitution, the IARS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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