Hydroxysteroid 17-beta Dehydrogenase 13 Antibody HSD17B13/13108 / Protein Microarray Validated [clone HSD17B13/13108] (V5743)

Hydroxysteroid 17-beta Dehydrogenase 13 Antibody HSD17B13/13108 Protein Microarray Validation. Protein microarray analysis of Hydroxysteroid 17-beta Dehydrogenase 13 Antibody, clone HSD17B13/13108, using a HuProt array containing more than 19,000 full-length human proteins. The intended HSD17B13 target produced the dominant signal, with a Z-score of 18.25 and an S-score of 18.17. The Z-score measures signal strength in standard deviations above the array mean, while the S-score measures the separation between the intended target and the next-highest protein signal. These results provide proteome-scale evidence supporting the target selectivity of this Protein Microarray Validated monoclonal antibody.

Hydroxysteroid 17-beta Dehydrogenase 13 Antibody Staining of Human Hepatocellular Carcinoma IHC. Immunohistochemistry of FFPE human hepatocellular carcinoma tissue using Hydroxysteroid 17-beta Dehydrogenase 13 Antibody, clone HSD17B13/13108. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before staining. Brown cytoplasmic immunoreactivity is visible throughout the tumor tissue. The inset shows the secondary antibody negative control, with PBS substituted for the primary antibody.

Purified Hydroxysteroid 17-beta Dehydrogenase 13 Antibody HSD17B13/13108 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Hydroxysteroid 17-beta Dehydrogenase 13 Antibody, clone HSD17B13/13108, with 2 ug loaded under non-reducing (NR) and reducing (R) conditions. The intact antibody migrates predominantly near 150 kDa under non-reducing conditions. Following reduction, the predominant heavy-chain band is visible near 50 kDa; the light-chain region is faint at approximately 25 kDa. The observed banding pattern supports the integrity and purity of the monoclonal antibody preparation.