HuR Antibody / Human Antigen R Antibody (F51453)

HuR Antibody Human Cell WB. Western blot analysis of HuR Antibody using lysates from 1) human HeLa cells, 2) human Jurkat cells, 3) human CCRF-CEM cells, and 4) human Ramos cells. A single, specific band is detected at approximately 32–36 kDa in all four cell lines, consistent with the expected molecular weight of HuR (ELAVL1). These results demonstrate that HuR Antibody, a Human Antigen R Antibody, specifically detects endogenous HuR across multiple human epithelial, T-cell leukemia, and B-cell lymphoma cell lines.

HuR Antibody Human Brain IHC. Immunohistochemical analysis of FFPE human brain tissue using HuR Antibody. Heat-induced epitope retrieval was performed prior to staining with HuR Antibody, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. Distinct nuclear staining is observed in neurons with lighter cytoplasmic immunoreactivity, consistent with the characteristic subcellular localization of HuR (ELAVL1) as an RNA-binding protein that primarily resides in the nucleus while undergoing regulated nucleocytoplasmic shuttling. These results demonstrate that HuR Antibody, a Human Antigen R Antibody, specifically detects endogenous HuR in FFPE human brain tissue.

HuR Antibody Jurkat Cell FACS. Flow cytometric analysis of Jurkat cells using HuR Antibody. Cells were incubated with HuR Antibody followed by an FITC-conjugated goat anti-rabbit secondary antibody. The upper histogram represents the negative control, while the lower histogram shows HuR-stained Jurkat cells. A clear rightward shift of the HuR-stained population relative to the negative control demonstrates specific detection of endogenous HuR (ELAVL1). These results demonstrate that HuR Antibody, a Human Antigen R Antibody, is suitable for flow cytometric analysis of HuR expression in human Jurkat cells.

HuR Antibody ELAVL1 Overexpression WB. Western blot analysis of HuR Antibody using lysates from 1) non-transfected human 293 cells and 2) human 293 cells transiently transfected with the ELAVL1 gene (2 ug/lane). A strong band is detected at approximately 32-36 kDa in the ELAVL1-transfected cells, while little to no signal is observed in the non-transfected control, consistent with the expected molecular weight of HuR. These results demonstrate that HuR Antibody, a Human Antigen R Antibody, specifically detects recombinant ELAVL1 and confirms its suitability for monitoring HuR overexpression by Western blot.