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Home >> Antibodies >> Human Nuclear Antigen Antibody

Human Nuclear Antigen Antibody [clone 235-1] (V2345CF488)

  Catalog No Formulation Size Price (USD)  
Image V2345CF488-100T 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 Tests 519
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Intracellular flow cytometry testing of human 293T cells with Human Nuclear Antigen antibody; Black=cells alone, Gray=isotype control, Green= Human Nuclear Antigen antibody.
Availability 1-3 business days
Species Reactivity Human
Format CF488 Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 235-1
Purity Protein G affinity chromatography
Localization Nuclear
Applications Flow cytometry : 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood
Immunofluorescence : 1-2ug/ml
Limitations This Human Nuclear Antigen antibody is available for research use only.
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Description

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Application Notes

Optimal dilution of the Human Nuclear Antigen antibody should be determined by the researcher.

Immunogen

Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.

Storage

Store the Human Nuclear Antigen antibody at 2-8oC, protected from light.

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