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Home >> Antibodies >> Human IgG4 Antibody

Human IgG4 Antibody [clone DCF-9] (RQ4982)

  Catalog No Formulation Size Price (USD)  
Image RQ4982 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human spleen lysate with Human IgG4 antibody. Predicted molecular weight ~50 kDa.
IHC staining of FFPE human stomach carcinoma with Human IgG4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name DCF-9
Purity Affinity purified
UniProt P01861
Applications Western blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:500-1:1000
Limitations This Human IgG4 antibody is available for research use only.
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Description

Gamma 4 constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. [UniProt]

Application Notes

Optimal dilution of the Human IgG4 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to Human IgG4 / IGHG4 was used as the immunogen for the IgG4 antibody.

Storage

Store the Human IgG4 antibody at -20oC.

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