HSPH1 Antibody / Heat Shock Protein Family H Member 1 Antibody [clone r58F12] (V5854)

HSPH1 Antibody Human Lung, A431 and Raji Cell WB. Western blot analysis of human lung tissue, A431 epidermoid carcinoma cells and Raji lymphoma cells was performed with HSPH1 Antibody, also called Heat Shock Protein Family H Member 1 Antibody, clone r58F12. A prominent band is detected at approximately 105 kDa across all tested samples. Although the sequence-predicted molecular weight of HSPH1 is approximately 97 kDa, HSP105 commonly exhibits an apparent molecular weight of approximately 105 kDa by SDS-PAGE. This HSPH1 Antibody demonstrates detection of Heat Shock Protein Family H Member 1 in human tissue and cell lysates.

HSPH1 Antibody Human Testis IHC. Immunohistochemistry staining of FFPE human testis tissue was performed with HSPH1 Antibody, also called Heat Shock Protein Family H Member 1 Antibody, clone r58F12. Nuclear and cytoplasmic staining is observed in a subset of testicular cells, with minimal staining of surrounding stromal elements. Heat-induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9, at 95oC for 45 min followed by cooling at RT for 20 min. The inset shows a secondary-only negative control in which PBS was substituted for the primary antibody.

HSPH1 Antibody r58F12 Purity by SDS-PAGE. SDS-PAGE analysis of purified recombinant mouse monoclonal HSPH1 Antibody clone r58F12 was performed under non-reducing (NR) and reducing (R) conditions with 2 ug of antibody loaded per lane. The non-reduced sample shows the intact antibody at approximately 150 kDa, while reduction produces bands corresponding to the approximately 50 kDa heavy chain and 25 kDa light chain. The results confirm the purity and structural integrity of the recombinant HSPH1 Antibody.