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Home >> Antibodies >> HSPB1 Antibody [Discontinued, view alternative antibodies]

HSPB1 Antibody [Discontinued, view alternative antibodies] (R30899)

  Catalog No Formulation Size Price (USD)  
Image R30899 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) human Caco-2 and 4) human Jurkat cell lysate with HSPB1 antibody. Expected molecular weight: 23~27 kDa.
ICC staining of FFPE human A549 cells with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung cancer with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat heart with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P04792
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This HSPB1 antibody is available for research use only.
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Description

HSPB1 (Heat shock 27kDa protein 1) is a protein that in humans is encoded by the HSPB1 gene. Stock et al.(2003) used FISH to map the HSP27 gene to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the HSPB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of human HSP27 (KHEERQDEHGYISRCFTR) was used as the immunogen for this HSPB1 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the HSPB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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