HSPA5 Antibody / Heat Shock Protein Family A Member 5 Antibody (F54266)

HSPA5 Antibody Human Leukemia Cell WB. Western blot analysis was performed using human HL-60 promyelocytic leukemia cell lysate. The HSPA5 antibody detected a distinct band within the expected 70-78 kDa migration range. The calculated molecular weight of HSPA5 is approximately 73 kDa, although variation within this range is routinely observed for the endogenous protein. The clean band supports specific detection of HSPA5 in a human hematopoietic cancer cell model. This HSPA5 Antibody, also described as a Heat Shock Protein Family A Member 5 Antibody, supports studies of protein folding, endoplasmic reticulum stress and unfolded protein response signaling.

HSPA5 Antibody Mouse Liver WB. Western blot analysis was performed using mouse liver tissue lysate. The HSPA5 antibody detected a distinct band within the expected 70-78 kDa migration range. The calculated molecular weight of HSPA5 is approximately 73 kDa, although endogenous HSPA5 is routinely observed across this slightly broader range. The result supports detection of mouse HSPA5 in metabolically and secretory active liver tissue. This HSPA5 Antibody, also described as a Heat Shock Protein Family A Member 5 Antibody, supports research into hepatic protein folding, endoplasmic reticulum homeostasis and unfolded protein response signaling.

HSPA5 Antibody Human Lung Cancer Cell IF. HSPA5 was detected in fixed and permeabilized human NCI-H460 lung cancer cells by immunofluorescence. HSPA5 antibody staining appears green, while nuclei were counterstained with DAPI and appear blue. The target displays a granular and reticular cytoplasmic distribution with prominent perinuclear signal, consistent with localization of HSPA5 within the endoplasmic reticulum. Nuclear staining is minimal. This HSPA5 Antibody, also described as a Heat Shock Protein Family A Member 5 Antibody, supports cellular localization studies involving protein folding, endoplasmic reticulum homeostasis and stress-response signaling in lung cancer cells.

HSPA5 Antibody Human Testis IHC. HSPA5 expression was examined in a formalin-fixed, paraffin-embedded section of human testis tissue. Heat-induced epitope retrieval was performed by steaming the section in citrate buffer, pH 6.0, for 20 minutes followed by cooling before staining. Red chromogenic signal is concentrated in a subset of cells within the seminiferous tubule, demonstrating variable HSPA5 expression among testicular cell populations. This HSPA5 Antibody, also described as a Heat Shock Protein Family A Member 5 Antibody, supports studies of endoplasmic reticulum protein folding and cellular stress responses in human reproductive tissue.

HSPA5 Antibody Human Prostate Carcinoma IHC. HSPA5 was detected in a formalin-fixed, paraffin-embedded section of human prostate carcinoma tissue. Heat-induced epitope retrieval was performed by steaming the section in citrate buffer, pH 6.0, for 20 minutes followed by cooling before staining. Brown chromogenic signal demonstrates strong granular cytoplasmic HSPA5 expression in malignant prostate epithelial cells, with variable intensity across the examined field. This HSPA5 Antibody, also described as a Heat Shock Protein Family A Member 5 Antibody, supports studies of endoplasmic reticulum protein-folding demand, cellular stress adaptation and unfolded protein response activity in prostate cancer.

HSPA5 Antibody Human Cervical Cancer Cell FACS. Intracellular flow cytometry analysis was performed using fixed and permeabilized human HeLa cervical cancer cells. The blue trace represents the isotype control, while the green trace represents cells stained with HSPA5 antibody. The pronounced rightward shift of the HSPA5-stained population relative to the isotype control demonstrates strong intracellular HSPA5 expression in HeLa cells. This HSPA5 Antibody, also described as a Heat Shock Protein Family A Member 5 Antibody, supports quantitative analysis of endoplasmic reticulum chaperone expression and unfolded protein response activity in human cancer cells.