HSPA1A Antibody / Heat Shock Protein 70 1A Antibody [clone HSPA1A/7936] (V5143)

HSPA1A Antibody HSPA1A/7936 Detection in Human Tissues and Cell Lines WB. Western blot analysis of human brain, human skeletal muscle, HeLa, MCF7, Raji and Ramos lysates using HSPA1A Antibody clone HSPA1A/7936 shows a strong, discrete band at approximately 70 kDa in all six samples, consistent with the expected molecular weight of HSPA1A. Minimal background and the consistent band size across tissue and cell lysates demonstrate clean Western blot detection of HSPA1A.

HSPA1A Antibody HSPA1A/7936 Detection in HEK-293 Cells WB. Western blot analysis of human HEK-293 cell lysate using HSPA1A Antibody clone HSPA1A/7936 shows a strong predominant band at approximately 70-75 kDa, consistent with the expected molecular weight of HSPA1A. Weaker lower molecular weight bands are also visible at approximately 50 kDa and 27-30 kDa.

HSPA1A Antibody HSPA1A/7936 Staining of Human Kidney IHC. Immunohistochemistry testing of FFPE human kidney tissue with HSPA1A Antibody clone HSPA1A/7936 shows strong brown staining throughout the renal tubular epithelium, while the inset secondary antibody negative control shows minimal background signal. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

Purified HSPA1A Antibody HSPA1A/7936 SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free HSPA1A Antibody clone HSPA1A/7936 under non-reducing (NR) and reducing (R) conditions. The non-reduced antibody migrates predominantly above 100 kDa, while reduction produces the expected immunoglobulin heavy and light chain bands at approximately 50 and 25 kDa, respectively, confirming antibody integrity and purity.