Hsp90 beta Antibody / Heat Shock Protein 90 Beta Antibody [clone 7B7F5] (RQ7569)

Hsp90 beta Antibody MCF-7 Cell IF. Immunofluorescence analysis of FFPE human MCF-7 cells using Hsp90 beta Antibody. Heat induced antigen retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Hsp90 beta is detected in green, with DAPI (blue) used as a nuclear counterstain. Strong diffuse cytoplasmic staining is observed throughout the cells, consistent with the constitutive cytosolic localization of HSP90AB1 as an ATP-dependent molecular chaperone involved in protein folding and protein homeostasis. These results support the use of Hsp90 beta Antibody / Heat Shock Protein 90 Beta Antibody for studying HSP90AB1 expression, molecular chaperone function and cellular stress responses.

Hsp90 beta Antibody Human, Mouse and Rat Tissue WB. Western blot analysis using Hsp90 beta Antibody. Lane 1: human HeLa; Lane 2: human Jurkat; Lane 3: human Raji; Lane 4: human A431; Lane 5: rat brain; Lane 6: rat heart; Lane 7: mouse brain; Lane 8: mouse heart lysate. A specific band is detected at approximately 84-90 kDa in all samples, consistent with the expected molecular weight of HSP90AB1. The broad expression profile across human, rat and mouse tissues reflects the constitutive nature of this ATP-dependent molecular chaperone. These results support the use of Hsp90 beta Antibody / Heat Shock Protein 90 Beta Antibody for studying HSP90AB1 expression, protein folding and cellular protein homeostasis.

Hsp90 beta Antibody Caco-2 Cell FACS. Flow cytometry analysis of fixed and permeabilized human Caco-2 cells using Hsp90 beta Antibody at 1 ug/million cells. Cells were blocked with goat serum prior to staining. Red = cells alone; Green = isotype control; Blue = Hsp90 beta Antibody. A distinct rightward shift of the antibody-stained population relative to the isotype control demonstrates specific detection of intracellular HSP90AB1. These results support the use of Hsp90 beta Antibody / Heat Shock Protein 90 Beta Antibody for flow cytometric analysis of HSP90AB1 expression, molecular chaperone function and cellular protein homeostasis.

Hsp90 beta Antibody Human Laryngeal Squamous Cell Carcinoma IHC. Immunohistochemistry analysis of FFPE human laryngeal squamous cell carcinoma tissue using Hsp90 beta Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-mouse IgG and DAB chromogen were used for visualization. Strong predominantly cytoplasmic staining is observed in malignant squamous epithelial cells, consistent with the intracellular localization of HSP90AB1 as a constitutively expressed molecular chaperone. These results support the use of Hsp90 beta Antibody for studying HSP90AB1, molecular chaperone function and cancer-associated protein homeostasis.

Hsp90 beta Antibody Human Thyroid Cancer IHC. Immunohistochemistry analysis of FFPE human thyroid cancer tissue using Hsp90 beta Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-mouse IgG and DAB chromogen were used for visualization. Predominantly cytoplasmic staining is observed in thyroid epithelial tumor cells, consistent with the intracellular localization of HSP90AB1 as a constitutively expressed molecular chaperone. These results support the use of Hsp90 beta Antibody for studying HSP90AB1, molecular chaperone function and cancer-associated protein homeostasis.

Hsp90 beta Antibody Human Placenta IHC. Immunohistochemistry analysis of FFPE human placenta tissue using Hsp90 beta Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-mouse IgG and DAB chromogen were used for visualization. Predominantly cytoplasmic staining is observed in placental trophoblast cells, consistent with the intracellular localization of HSP90AB1 as a constitutively expressed molecular chaperone. These results support the use of Hsp90 beta Antibody for studying HSP90AB1, molecular chaperone function and cellular protein homeostasis.

Hsp90 beta Antibody Mouse Brain IHC. Immunohistochemistry analysis of FFPE mouse brain tissue using Hsp90 beta Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-mouse IgG and DAB chromogen were used for visualization. Predominantly cytoplasmic staining is observed throughout neuronal cell populations, consistent with the constitutive expression of HSP90AB1 as a molecular chaperone involved in protein folding and cellular stress responses. These results support the use of Hsp90 beta Antibody for studying HSP90AB1, molecular chaperone function and nervous system biology.

Hsp90 beta Antibody Rat Brain IHC. Immunohistochemistry analysis of FFPE rat brain tissue using Hsp90 beta Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-mouse IgG and DAB chromogen were used for visualization. Predominantly cytoplasmic staining is observed in neuronal cell populations, consistent with the constitutive expression of HSP90AB1 as a molecular chaperone involved in protein folding and cellular stress responses. These results support the use of Hsp90 beta Antibody for studying HSP90AB1, molecular chaperone function and nervous system biology.