HSP90 alpha/beta Antibody / HSP90AA1 + HSP90AB1 [clone EBD-8] (RQ5187)

HSP90 alpha/beta Antibody / HSP90AA1 + HSP90AB1 in Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue with HSP90 alpha/beta Antibody clone EBD-8. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before testing. Positive HSP90 alpha/beta staining is detected within the tumor tissue.

HSP90 alpha/beta Antibody in Human Colorectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human colorectal adenocarcinoma tissue with HSP90 alpha/beta Antibody clone EBD-8 shows strong cytoplasmic staining throughout the malignant glandular epithelium. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before testing.

HSP90 alpha/beta Antibody in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with HSP90 alpha/beta Antibody clone EBD-8 shows widespread cytoplasmic staining of tumor cells, with heterogeneous staining intensity across the section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before testing.

HSP90 alpha/beta Antibody in Human Lymphoma IHC. Immunohistochemistry staining of FFPE human lymphoma tissue with HSP90 alpha/beta Antibody clone EBD-8 shows widespread cytoplasmic staining among the lymphoid cells. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before testing.

HSP90 alpha/beta Antibody in Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with HSP90 alpha/beta Antibody clone EBD-8 shows diffuse staining throughout the brain parenchyma, including neuronal cell bodies and surrounding neuropil. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before testing.

HSP90 alpha/beta Antibody in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with HSP90 alpha/beta Antibody clone EBD-8 shows broad staining across the brain parenchyma, including neuronal cell bodies and surrounding neuropil. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before testing.

HSP90 alpha/beta Antibody in HeLa Cervical Cancer Cells IF. Immunofluorescence staining of FFPE human HeLa cervical cancer cells with HSP90 alpha/beta Antibody clone EBD-8 detects HSP90 alpha/beta protein (green), while DAPI counterstaining identifies nuclei (blue). Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 min.

HSP90 alpha/beta Antibody in HeLa Cervical Cancer Cells IF. Immunofluorescence staining of FFPE human HeLa cervical cancer cells with HSP90 alpha/beta Antibody clone EBD-8 shows green HSP90 alpha/beta signal predominantly outside the DAPI-stained nuclei, consistent with substantial cytoplasmic localization. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 min.

HSP90 alpha/beta Antibody Multi-Species Expression WB. Western blot testing of human 293T, U-251 and SiHa cell lysates, rat brain lysate, mouse brain lysate and mouse kidney lysate with HSP90 alpha/beta Antibody clone EBD-8. A strong predominant band is detected at approximately 84-90 kDa in all six lanes, consistent with the expected molecular weight range of cytosolic HSP90 proteins. The results demonstrate detection across human, rat and mouse samples.

HSP90 alpha/beta Antibody in MDA-MB-231 and HCC1937 Breast Cancer Cells WB. Western blot testing of human MDA-MB-231 and HCC1937 breast cancer cell lysates with HSP90 alpha/beta Antibody shows a strong, specific band at approximately 90 kDa, consistent with the expected molecular weight of HSP90AA1. Samples were separated on an 8% SDS-PAGE gel under reducing conditions, transferred to nitrocellulose, and probed with the antibody at 0.5 ug/ml overnight at 4oC. GAPDH at approximately 35 kDa serves as a loading control and shows comparable protein loading across the four lanes.