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Home >> Antibodies >> HSP90 alpha/beta Antibody

HSP90 alpha/beta Antibody [clone EBD-8] (RQ5187)

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Image RQ5187 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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IHC staining of FFPE human breast cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with HSP90 alpha/beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with HSP90 alpha/beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human U-251, 3) human SiHa, 4) rat brain, 5) mouse brain and 6) mouse kidney tissue lysate with HSP90 alpha/beta antibody. Predicted molecular weight: 84-90 kDa.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name EBD-8
Purity Affinity purified
UniProt P07900
Localization Cell membrane, cytoplasmic, nuclear
Applications Western blot : 1:5000-1:10000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50-1:200
Limitations This HSP90 alpha/beta antibody is available for research use only.
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Description

Heat shock protein 90 (Hsp90) is one of the most common of the heat-related proteins. Hsp90 is found in bacteria and all branches of eukarya, but it is apparently absent in archaea. Whereas cytoplasmic Hsp90 is essential for viability under all conditions in eukaryotes, the bacterial homologue HtpG is dispensable under non-heat stress conditions. [Wiki]

Application Notes

Optimal dilution of the HSP90 alpha/beta antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human HSP90 alpha/beta was used as the immunogen for the HSP90 alpha/beta antibody.

Storage

Store the HSP90 alpha/beta antibody at -20oC.

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