HSP72 Antibody | Inducible HSP70 Antibody

	Catalog No	Formulation	Size	Price (USD)
	R30911	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

HSP72 Antibody Detection in Rat and Mouse Samples WB. Western blot testing of rat liver (lane 1), kidney (lane 2), brain (lane 3) and RH35 (lane 4), and mouse liver (lane 5), kidney (lane 6), brain (lane 7) and NIH 3T3 (lane 8) lysates with HSP72 Antibody shows a prominent band at approximately 70 kDa across all samples, consistent with HSPA1A/HSPA1B. Additional lower molecular weight bands are visible in several lanes.

HSP72 Antibody Staining of Human Intestinal Cancer IHC. Immunohistochemistry testing of FFPE human intestinal cancer tissue with HSP72 Antibody shows brown immunoreactivity within the malignant glandular epithelium, with predominantly cytoplasmic staining and comparatively less staining in surrounding stromal areas.

Inducible HSP70 Antibody Staining of A549 Lung Cancer Cells IF. Immunofluorescence staining of human A549 lung cancer cells with Inducible HSP70 Antibody shows prominent brown cytoplasmic and perinuclear immunoreactivity, with blue-counterstained nuclei.

HSP72 Antibody Staining of Rat Intestine IHC. Immunohistochemistry testing of frozen rat intestine tissue with HSP72 Antibody shows brown immunoreactivity within the intestinal epithelium, with particularly prominent staining toward the luminal surface.

HSP72 Antibody Detection in Human Cancer Cell Lines WB. Western blot testing of human HeLa (lane 1), HepG2 (lane 2), A431 (lane 3), MCF7 (lane 4), A549 (lane 5), PC-3 (lane 6), 293T (lane 7) and K562 (lane 8) cell lysates with HSP72 Antibody shows strong, discrete bands at approximately 70 kDa across all eight samples, consistent with the expected molecular weight of the inducible HSPA1A/HSPA1B proteins.

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HSP72 Antibody Detection in Rat and Mouse Samples WB. Western blot testing of rat liver (lane 1), kidney (lane 2), brain (lane 3) and RH35 (lane 4), and mouse liver (lane 5), kidney (lane 6), brain (lane 7) and NIH 3T3 (lane 8) lysates with HSP72 Antibody shows a prominent band at approximately 70 kDa across all samples, consistent with HSPA1A/HSPA1B. Additional lower molecular weight bands are visible in several lanes.

HSP72 Antibody Staining of Human Intestinal Cancer IHC. Immunohistochemistry testing of FFPE human intestinal cancer tissue with HSP72 Antibody shows brown immunoreactivity within the malignant glandular epithelium, with predominantly cytoplasmic staining and comparatively less staining in surrounding stromal areas.

Inducible HSP70 Antibody Staining of A549 Lung Cancer Cells IF. Immunofluorescence staining of human A549 lung cancer cells with Inducible HSP70 Antibody shows prominent brown cytoplasmic and perinuclear immunoreactivity, with blue-counterstained nuclei.

HSP72 Antibody Staining of Rat Intestine IHC. Immunohistochemistry testing of frozen rat intestine tissue with HSP72 Antibody shows brown immunoreactivity within the intestinal epithelium, with particularly prominent staining toward the luminal surface.

HSP72 Antibody Detection in Human Cancer Cell Lines WB. Western blot testing of human HeLa (lane 1), HepG2 (lane 2), A431 (lane 3), MCF7 (lane 4), A549 (lane 5), PC-3 (lane 6), 293T (lane 7) and K562 (lane 8) cell lysates with HSP72 Antibody shows strong, discrete bands at approximately 70 kDa across all eight samples, consistent with the expected molecular weight of the inducible HSPA1A/HSPA1B proteins.

Availability	1-3 business days
Species Reactivity	Human, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt	P08107
Applications	Western Blot : 0.5-1ug/ml IHC (FFPE) : 0.5-1ug/ml IHC (Frozen) : 0.5-1ug/ml Immunocytochemistry : 0.5-1ug/ml
Limitations	This HSP72 Antibody / Inducible HSP70 Antibody is available for research use only.
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Description

HSP72 Antibody / Inducible HSP70 Antibody recognizes the closely related stress-inducible proteins HSPA1A and HSPA1B. These members of the HSP70 molecular chaperone family are strongly induced by heat shock and other forms of cellular stress. HSP72 is commonly used to describe the inducible HSP70 proteins and distinguish them from constitutively expressed members of the broader HSP70 family.

HSPA1A and HSPA1B encode highly homologous proteins that help maintain proteome integrity during normal cellular activity and periods of stress. HSP70 chaperones bind exposed hydrophobic regions of unfolded or partially folded proteins, limiting inappropriate interactions and aggregation. An ATP-dependent cycle controls substrate binding and release, while numerous co-chaperones regulate HSP70 activity, substrate selection and interactions with other components of the protein quality-control machinery.

Inducible HSP70 expression can increase rapidly in response to elevated temperature, oxidative stress, metabolic disruption and other conditions that compromise protein stability. This response provides cells with additional chaperone capacity at times when damaged or unfolded proteins accumulate. HSPA1A and HSPA1B can assist protein refolding and cooperate with cellular degradation systems when damaged proteins cannot be restored, making HSP72 an important component of the proteotoxic stress response.

HSP72 biology is also closely connected with cell survival and apoptosis. Increased inducible HSP70 activity can help cells tolerate otherwise damaging conditions by stabilizing proteins and influencing stress-response pathways. These functions have generated substantial interest in HSP72 in cancer research, where tumor cells frequently encounter hypoxia, oxidative stress, nutrient limitation and elevated demands on protein quality-control systems.

NSJ Bioreagents offers this rabbit polyclonal HSP72 Antibody generated against a peptide corresponding to amino acids 577-596 of human HSPA1A. The immunogen sequence is also present in HSPA1B and therefore does not distinguish between these two highly related inducible HSP70 proteins. An Inducible HSP70 Antibody provides a research tool for investigating HSPA1A/HSPA1B expression, molecular chaperone activity, proteostasis and cellular adaptation to stress.

For a monoclonal antibody recognizing the closely related HSPA1A and HSPA1B proteins, see our HSP70 Antibody / HSPA1A HSPA1B Antibody page.

Application Notes

The stated application concentrations are suggested starting points. Titration of the HSP72 Antibody / Inducible HSP70 Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 577-596 (VISWLDANTLAEKDEFEHKR-human) were used as the immunogen for this HSP72 antibody.

Storage

After reconstitution, the HSP72 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

HSPA1A antibody, HSPA1B antibody, HSP70 antibody, Heat shock protein 70 antibody, HSP70-1 antibody, HSPA1 antibody

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HSP72 Antibody / Inducible HSP70 Antibody (R30911)

Description

Application Notes

Immunogen

Storage

Alternate Names

Related Products

Area of Research

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Protocols

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NSJ Bioreagents