HSP70-1A Antibody | Heat Shock 70 kDa Protein 1A

	Catalog No	Formulation	Size	Price (USD)
	V5142-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V5142-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V5142SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

HSP70-1A Antibody HSPA1A/7932 Detection in Human Tissues and Cell Lines WB. Western blot analysis of human brain, human skeletal muscle, HeLa, MCF7, Raji and Ramos lysates using HSP70-1A Antibody clone HSPA1A/7932 shows a strong, discrete band at approximately 70 kDa in all six samples, consistent with the expected molecular weight of HSPA1A. The consistent band size and minimal background demonstrate clean Western blot detection across human tissue and cell lysates.

HSP70-1A Antibody HSPA1A/7932 Detection in HEK-293 Cells WB. Western blot analysis of human HEK-293 cell lysate using HSP70-1A Antibody clone HSPA1A/7932 shows a strong predominant band at approximately 70-75 kDa, consistent with the expected molecular weight of HSPA1A. A weaker band is visible immediately below the principal signal.

HSP70-1A Antibody HSPA1A/7932 Staining of Human Testis IHC. Immunohistochemistry testing of FFPE human testis tissue with HSP70-1A Antibody clone HSPA1A/7932 shows strong brown immunoreactivity within the seminiferous tubules, with widespread cytoplasmic staining. The inset secondary antibody negative control shows minimal background signal. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

HSP70-1A Antibody HSPA1A/7932 Staining of Human Kidney IHC. Immunohistochemistry testing of FFPE human kidney tissue with HSP70-1A Antibody clone HSPA1A/7932 shows strong brown immunoreactivity in renal tubular epithelial cells, with comparatively less staining in glomerular structures. The inset secondary antibody negative control shows minimal background signal. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

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HSP70-1A Antibody HSPA1A/7932 Detection in Human Tissues and Cell Lines WB. Western blot analysis of human brain, human skeletal muscle, HeLa, MCF7, Raji and Ramos lysates using HSP70-1A Antibody clone HSPA1A/7932 shows a strong, discrete band at approximately 70 kDa in all six samples, consistent with the expected molecular weight of HSPA1A. The consistent band size and minimal background demonstrate clean Western blot detection across human tissue and cell lysates.

HSP70-1A Antibody HSPA1A/7932 Detection in HEK-293 Cells WB. Western blot analysis of human HEK-293 cell lysate using HSP70-1A Antibody clone HSPA1A/7932 shows a strong predominant band at approximately 70-75 kDa, consistent with the expected molecular weight of HSPA1A. A weaker band is visible immediately below the principal signal.

HSP70-1A Antibody HSPA1A/7932 Staining of Human Testis IHC. Immunohistochemistry testing of FFPE human testis tissue with HSP70-1A Antibody clone HSPA1A/7932 shows strong brown immunoreactivity within the seminiferous tubules, with widespread cytoplasmic staining. The inset secondary antibody negative control shows minimal background signal. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

HSP70-1A Antibody HSPA1A/7932 Staining of Human Kidney IHC. Immunohistochemistry testing of FFPE human kidney tissue with HSP70-1A Antibody clone HSPA1A/7932 shows strong brown immunoreactivity in renal tubular epithelial cells, with comparatively less staining in glomerular structures. The inset secondary antibody negative control shows minimal background signal. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

Purified HSP70-1A Antibody HSPA1A/7932 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free HSP70-1A Antibody clone HSPA1A/7932 under non-reducing and reducing conditions demonstrates the expected electrophoretic profile of purified mouse IgG. Reduction produces the characteristic immunoglobulin heavy and light chain bands at approximately 50 and 25 kDa, respectively, supporting antibody integrity and purity.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat, Hamster, Rabbit, Guinea pig
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	HSPA1A/7932
Purity	Protein A/G affinity
UniProt	P0DMV8
Localization	Cytoplasm
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (Human) Western Blot : 2-4ug/ml (Human/Mouse/Rat/Hamster/Rabbit/Guinea pig)
Limitations	This HSP70-1A Antibody / Heat Shock 70 kDa Protein 1A Antibody is available for research use only.
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Description

HSP70-1A Antibody / Heat Shock 70 kDa Protein 1A Antibody recognizes HSPA1A, a stress-inducible molecular chaperone belonging to the HSP70 protein family. HSPA1A is commonly called HSP70-1A and is rapidly induced when cellular conditions disrupt normal protein folding. Its increased expression provides additional chaperone capacity that helps protect cells against proteotoxic stress and maintain protein homeostasis.

HSPA1A is an approximately 70 kDa protein composed of an N-terminal nucleotide-binding domain and a C-terminal substrate-binding domain. Its chaperone activity is driven by an ATP-dependent cycle that regulates the binding and release of client proteins. Co-chaperones further control this cycle by influencing nucleotide exchange, ATP hydrolysis and substrate interactions. These coordinated activities enable HSP70-1A to recognize exposed regions of unfolded or partially folded proteins, limit inappropriate aggregation and facilitate productive refolding.

Heat Shock 70 kDa Protein 1A is an important component of the cellular response to heat shock, oxidative stress and other conditions that challenge proteome integrity. HSPA1A also interacts functionally with protein degradation systems when damaged proteins cannot be successfully restored. This relationship between molecular chaperoning, protein refolding and degradation allows cells to adjust protein quality-control capacity in response to changing physiological conditions.

HSPA1A has also been studied extensively in cancer biology. Tumor cells can experience hypoxia, nutrient limitation, oxidative stress and elevated protein synthesis, all of which increase demands on protein quality-control machinery. Increased HSPA1A activity may promote cellular adaptation to these conditions and has been investigated in relation to tumor cell survival, apoptosis and responses to therapeutic stress.

NSJ Bioreagents offers this mouse monoclonal HSP70-1A Antibody as clone HSPA1A/7932. The antibody was generated using a recombinant region of human HSPA1A within amino acids 200-400. Because HSPA1A and HSPA1B are highly homologous and the precise epitope recognized by clone HSPA1A/7932 is not disclosed, the immunogen information alone does not establish exclusion of HSPA1B. A Heat Shock 70 kDa Protein 1A Antibody provides a research tool for studying HSPA1A biology, molecular chaperone function, proteostasis and cellular stress responses.

For an additional monoclonal antibody to HSPA1A with Western blot and immunohistochemistry validation, see our HSPA1A Antibody / Heat Shock Protein 70 1A Antibody page.

Application Notes

Optimal dilution of the HSP70-1A Antibody / Heat Shock 70 kDa Protein 1A Antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the HSP70-1A antibody.

Storage

Aliquot the HSP70-1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

HSPA1A antibody, Heat shock 70 kDa protein 1A antibody, Heat shock protein 70 1A antibody, HSP72 antibody

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HSP70-1A Antibody / Heat Shock 70 kDa Protein 1A Antibody [clone HSPA1A/7932] (V5142)

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