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Home >> Antibodies >> HSP60 Antibody / GROEL

HSP60 Antibody / GROEL [clone HSPD1/8398R] (V4931)

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  Catalog No Formulation Size Price (USD)  
Image V4931-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4931-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4931SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human kidney tissue with HSP60 antibody (clone HSPD1/8398R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human kidney tissue with HSP60 antibody (clone HSPD1/8398R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free HSP60 antibody (clone HSPD1/8398R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name HSPD1/8398R
Purity Protein A/G affinity
UniProt P10809
Localization Mitochondria
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This HSP60 antibody is available for research use only.
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Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/8398R, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Application Notes

Optimal dilution of the HSP60 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length HSP60 protein was used as the immunogen for the HSP60 antibody.

Storage

Aliquot the HSP60 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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