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Home >> Antibodies >> Hsp60 Antibody

Hsp60 Antibody [clone 6G2] (RQ5508)

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  Catalog No Formulation Size Price (USD)  
Image RQ5508 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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IHC staining of FFPE human breast cancer with Hsp60 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with Hsp60 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Hsp60 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with Hsp60 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) Caco-2, 2) A549, 3) ThP-1, 4) SW620, 5) U-937, 6) HepG2, 7) rat RH30 and 8) mouse RAW246.7 lysate with Hsp60 antibody. Predicted molecular weight ~60 kDa.
Flow cytometry testing of human A431 cells with Hsp60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hsp60 antibody.
Flow cytometry testing of human HepG2 cells with Hsp60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hsp60 antibody.
IHC staining of FFPE mouse liver with Hsp60 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver with Hsp60 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 6G2
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P10809
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This Hsp60 antibody is available for research use only.
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Description

HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Application Notes

Optimal dilution of the Hsp60 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids A260-Q496) was used as the immunogen for the Hsp60 antibody.

Storage

After reconstitution, the Hsp60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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