HSP47 Antibody / Collagen Chaperone Antibody (R31738)

HSP47 Antibody Human, Mouse and Rat WB. Western blot analysis was performed using HSP47 Antibody on lysates from lane 1: human A431, lane 2: human SK-N-SH, lane 3: human HeLa, lane 4: rat lung, lane 5: rat kidney, lane 6: rat L6, lane 7: mouse lung, and lane 8: mouse NIH 3T3 cells. A prominent immunoreactive band is detected at approximately 46 kDa, consistent with the predicted molecular weight of HSP47 (SERPINH1). Comparable detection across human, rat, and mouse samples demonstrates broad species reactivity. This HSP47 Antibody, a Collagen Chaperone Antibody, is well suited for Western blot studies of collagen biosynthesis, endoplasmic reticulum function, extracellular matrix remodeling, and fibrosis research.

HSP47 Antibody Human Esophageal Squamous Carcinoma IHC. HSP47 Antibody was used for immunohistochemical staining of FFPE human esophageal squamous carcinoma tissue following heat-induced epitope retrieval in pH 8 EDTA buffer. The tissue was incubated with the primary antibody, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. Strong cytoplasmic staining is observed in malignant squamous epithelial cells with additional labeling of stromal fibroblast-like cells, consistent with the endoplasmic reticulum localization and collagen chaperone function of SERPINH1. This HSP47 Antibody, a Collagen Chaperone Antibody, is well suited for studying collagen biosynthesis, extracellular matrix remodeling, fibrosis, and stromal responses in esophageal squamous carcinoma.

HSP47 Antibody Breast Cancer IHC. HSP47 Antibody was used for immunohistochemical staining of FFPE human breast cancer tissue following heat-induced epitope retrieval in pH 8 EDTA buffer. The section was incubated with the primary antibody at 2 ug/ml, followed by an HRP-conjugated goat anti-rabbit secondary antibody and DAB chromogen for visualization. Distinct cytoplasmic staining is observed within tumor-associated stromal cells and collagen-producing regions, consistent with the endoplasmic reticulum localization and collagen chaperone function of SERPINH1. This HSP47 Antibody, a Collagen Chaperone Antibody, is well suited for investigating extracellular matrix remodeling, collagen biosynthesis, fibrosis, and the stromal response associated with breast cancer.

HSP47 Antibody Human Breast Cancer Tissue IHC. HSP47 Antibody was used for immunohistochemical staining of FFPE human breast cancer tissue following heat-induced epitope retrieval in pH 8 EDTA buffer. The tissue was incubated with the primary antibody, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. Strong cytoplasmic staining is observed in malignant epithelial cells with additional labeling of stromal spindle-shaped cells, consistent with the endoplasmic reticulum localization and collagen chaperone function of SERPINH1. This HSP47 Antibody, a Collagen Chaperone Antibody, is well suited for investigating collagen biosynthesis, extracellular matrix remodeling, fibrosis, and the tumor microenvironment in breast cancer.

HSP47 Antibody Human Esophageal Squamous Carcinoma IHC. HSP47 Antibody was used for immunohistochemical staining of FFPE human esophageal squamous carcinoma tissue following heat-induced epitope retrieval in pH 8 EDTA buffer. The section was incubated with the primary antibody at 2 ug/ml, followed by an HRP-conjugated goat anti-rabbit secondary antibody and DAB chromogen for detection. Moderate to strong cytoplasmic staining is observed within malignant squamous epithelial cells and the surrounding stromal compartment, consistent with the expression pattern of the collagen-specific chaperone SERPINH1 during extracellular matrix remodeling. This HSP47 Antibody, a Collagen Chaperone Antibody, is useful for studying collagen biosynthesis, tumor-associated fibrosis, and stromal remodeling in esophageal cancer.

HSP47 Antibody Human Appendiceal Adenocarcinoma IHC. HSP47 Antibody was used for immunohistochemical staining of FFPE human appendiceal adenocarcinoma tissue following heat-induced epitope retrieval in pH 8 EDTA buffer. The section was incubated with the primary antibody at 2 ug/ml, followed by an HRP-conjugated goat anti-rabbit secondary antibody and DAB chromogen for detection. Prominent cytoplasmic staining is observed within stromal spindle-shaped cells and surrounding connective tissue, with weaker staining present in subsets of tumor cells, consistent with the role of SERPINH1 in collagen biosynthesis and extracellular matrix remodeling. This HSP47 Antibody, a Collagen Chaperone Antibody, is valuable for investigating fibrosis, collagen deposition, and stromal remodeling within the tumor microenvironment.

HSP47 Antibody Human Colon Cancer IHC. HSP47 Antibody was used for immunohistochemical staining of FFPE human colon cancer tissue following heat-induced epitope retrieval in pH 8 EDTA buffer. The section was incubated with the primary antibody at 2 ug/ml, followed by an HRP-conjugated goat anti-rabbit secondary antibody and DAB chromogen for detection. Predominant cytoplasmic staining is observed within stromal fibroblast-like cells surrounding the colonic glands, with comparatively weaker staining in the epithelial tumor cells, reflecting the collagen-associated expression pattern of SERPINH1 during extracellular matrix remodeling. This HSP47 Antibody, a Collagen Chaperone Antibody, is well suited for investigating collagen biosynthesis, tumor-associated fibrosis, and stromal remodeling in colorectal cancer.

HSP47 Antibody A549 Cell IF. HSP47 Antibody was used for immunofluorescent staining of A549 cells following enzyme-mediated antigen retrieval with AR0022 for 15 minutes. Cells were incubated with the primary antibody at 5 ug/ml, followed by an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody, while nuclei were counterstained with DAPI (blue). Bright green cytoplasmic fluorescence with a perinuclear distribution is observed, consistent with the localization of the collagen-specific chaperone SERPINH1 within the endoplasmic reticulum. This HSP47 Antibody, a Collagen Chaperone Antibody, is well suited for investigating collagen biosynthesis, endoplasmic reticulum function, extracellular matrix remodeling, and fibrosis-related cellular processes.

HSP47 Antibody A431 Cell FACS. HSP47 Antibody was evaluated by flow cytometry using fixed and permeabilized A431 cells. Cells were incubated with the primary antibody at 1 ug/1×10^6 cells, followed by a Fluoro488-conjugated goat anti-rabbit secondary antibody. The blue histogram shows a clear rightward shift relative to the rabbit IgG isotype control (green) and the unstained blank control (red), demonstrating specific intracellular detection of HSP47. This HSP47 Antibody, a Collagen Chaperone Antibody, is suitable for flow cytometric analysis of SERPINH1 expression in permeabilized cells and supports studies of collagen biosynthesis, endoplasmic reticulum function, and extracellular matrix remodeling.

HSP47 Antibody Human and Rodent Lung WB. Western blot analysis was performed using HSP47 Antibody on lane 1: human Caco-2, lane 2: human U2OS, lane 3: human HaCaT, lane 4: human SiHa, lane 5: rat lung, and lane 6: mouse lung lysates. A distinct band is detected at approximately 46 kDa in each sample, matching the predicted molecular weight of HSP47 (SERPINH1). The strong signal observed in both rat and mouse lung tissues is consistent with the abundant collagen synthesis occurring within pulmonary tissue. This HSP47 Antibody, a Collagen Chaperone Antibody, is well suited for Western blot studies of collagen biosynthesis, endoplasmic reticulum function, extracellular matrix remodeling, and fibrosis research.