HSP27 Antibody [clone SPM252] (V9024)

Description

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Application Notes

The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.

Storage

Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).