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Home >> Antibodies >> Hsp27 Antibody

Hsp27 Antibody (RQ4574)

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Image RQ4574 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat skeletal muscle, 2) rat lung and mouse 3) spleen, 4) skeletal muscle, 5) lung, 6) liver and 7) testis lysate with Hsp27 antibody at 0.5ug/ml. Predicted molecular weight: 23-27 kDa.
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Western blot testing of 1) rat skeletal muscle, 2) rat lung and mouse 3) spleen, 4) skeletal muscle, 5) lung, 6) liver and 7) testis lysate with Hsp27 antibody at 0.5ug/ml. Predicted molecular weight: 23-27 kDa.
IHC staining of FFPE mouse small intestine with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat small intestine with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat lung with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P14602
Localization Predominantly cytoplasmic with some nuclear
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 2-3ug/ml
Direct ELISA : 0.1-0.5ug/ml (recombinant mouse protein)
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Description

HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. It is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Application Notes

Optimal dilution of the Hsp27 antibody should be determined by the researcher.

Immunogen

Amino acids M1-K209 from the mouse protein were used as the immunogen for the Hsp27 antibody.

Storage

After reconstitution, the Hsp27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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