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Home >> Antibodies >> HSP27 Antibody

HSP27 Antibody [clone HSPB1/774] (V2593)

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  Catalog No Formulation Size Price (USD)  
Image V2593-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2593-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2593SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2593IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone HSPB1/774, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone HSPB1/774, green) and Reddot nuclear stain (red).
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone HSPB1/774).
IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with HSP27 antibody (clone HSPB1/774).
Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone HSPB1/774); Red=isotype control, Blue= HSP27 antibody.
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone HSPB1/774) as confirmation of integrity and purity.
Western blot testing of human HeLa cell lysate with HSP27 antibody. Expected molecular weight: 23~27 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name HSPB1/774
Purity Protein G affinity chromatography
UniProt P04792
Localization Predominantly cytoplasmic with some nuclear
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This HSP27 antibody is available for research use only.
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Description

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Application Notes

Optimal dilution of the HSP27 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human HSPB1 protein was used as the immunogen for the HSP27 antibody.

Storage

Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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