Hsp105 Antibody | Mouse Monoclonal | RQ5517 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ5517	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

HSP105 Antibody Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. Positive staining is observed in the malignant glandular epithelial cells. This HSP105 Antibody demonstrates HSPH1 expression in human intestinal cancer and supports studies of HSP70-associated chaperone activity, proteostasis and cancer biology.

HSP105 Antibody Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. Positive staining is observed in the malignant epithelial cells. This HSP105 Antibody demonstrates HSPH1 expression in human breast cancer and supports studies of HSP70-associated chaperone activity, proteostasis and cancer biology.

HSP105 Antibody Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. Positive staining is observed throughout the malignant epithelial cells. This HSP105 Antibody demonstrates HSPH1 expression in human lung cancer and supports studies of HSP70-associated chaperone activity, proteostasis and cancer biology.

HSP105 Antibody Human A431 Epidermoid Carcinoma Cell IF. Immunofluorescence staining of FFPE human A431 epidermoid carcinoma cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody (green), with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by boiling in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. HSP105 staining is observed predominantly in the cytoplasmic compartment. This HSP105 Antibody demonstrates HSPH1 localization in human A431 cells and supports studies of HSP70-associated chaperone activity and proteostasis.

HSP105 Antibody Human Cancer Cell WB. Western blot analysis was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody, using human 1) Caco-2, 2) K562, 3) A549, 4) HepG2, 5) PANC-1 and 6) SGC-7901 cell lysates. A strong band is detected at approximately 105-110 kDa across all tested samples, consistent with the expected molecular weight of HSP105/HSPH1. This HSP105 Antibody demonstrates detection of endogenous HSPH1 across multiple human cancer cell lines and supports studies of HSP70-associated chaperone activity and proteostasis.

HSP105 Antibody Human HepG2 Liver Cancer Cell FACS. Flow cytometry analysis of human HepG2 liver cancer cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody, at 1 ug per million cells following blocking with goat serum. The red histogram represents cells alone, green represents the isotype control, and blue represents HSP105 Antibody staining. A clear fluorescence shift is observed with the HSP105 Antibody compared with the isotype control, demonstrating detection of HSPH1 in human HepG2 cells.

HSP105 Antibody Human A431 Epidermoid Carcinoma Cell FACS. Flow cytometry analysis of human A431 epidermoid carcinoma cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody, at 1 ug per million cells following blocking with goat serum. The red histogram represents cells alone, green represents the isotype control, and blue represents HSP105 Antibody staining. A clear fluorescence shift is observed with the HSP105 Antibody compared with the isotype control, demonstrating detection of HSPH1 in human A431 cells.

HSP105 Antibody Human MCF-7 Breast Cancer Cell IF. Immunofluorescence staining of FFPE human MCF-7 breast cancer cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody (green), with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by boiling in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. HSP105 staining is observed predominantly in the cytoplasmic compartment. This HSP105 Antibody demonstrates HSPH1 localization in human MCF-7 cells and supports studies of HSP70-associated chaperone activity and proteostasis.

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HSP105 Antibody Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. Positive staining is observed in the malignant glandular epithelial cells. This HSP105 Antibody demonstrates HSPH1 expression in human intestinal cancer and supports studies of HSP70-associated chaperone activity, proteostasis and cancer biology.

HSP105 Antibody Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. Positive staining is observed in the malignant epithelial cells. This HSP105 Antibody demonstrates HSPH1 expression in human breast cancer and supports studies of HSP70-associated chaperone activity, proteostasis and cancer biology.

HSP105 Antibody Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. Positive staining is observed throughout the malignant epithelial cells. This HSP105 Antibody demonstrates HSPH1 expression in human lung cancer and supports studies of HSP70-associated chaperone activity, proteostasis and cancer biology.

HSP105 Antibody Human A431 Epidermoid Carcinoma Cell IF. Immunofluorescence staining of FFPE human A431 epidermoid carcinoma cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody (green), with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by boiling in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. HSP105 staining is observed predominantly in the cytoplasmic compartment. This HSP105 Antibody demonstrates HSPH1 localization in human A431 cells and supports studies of HSP70-associated chaperone activity and proteostasis.

HSP105 Antibody Human Cancer Cell WB. Western blot analysis was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody, using human 1) Caco-2, 2) K562, 3) A549, 4) HepG2, 5) PANC-1 and 6) SGC-7901 cell lysates. A strong band is detected at approximately 105-110 kDa across all tested samples, consistent with the expected molecular weight of HSP105/HSPH1. This HSP105 Antibody demonstrates detection of endogenous HSPH1 across multiple human cancer cell lines and supports studies of HSP70-associated chaperone activity and proteostasis.

HSP105 Antibody Human HepG2 Liver Cancer Cell FACS. Flow cytometry analysis of human HepG2 liver cancer cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody, at 1 ug per million cells following blocking with goat serum. The red histogram represents cells alone, green represents the isotype control, and blue represents HSP105 Antibody staining. A clear fluorescence shift is observed with the HSP105 Antibody compared with the isotype control, demonstrating detection of HSPH1 in human HepG2 cells.

HSP105 Antibody Human A431 Epidermoid Carcinoma Cell FACS. Flow cytometry analysis of human A431 epidermoid carcinoma cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody, at 1 ug per million cells following blocking with goat serum. The red histogram represents cells alone, green represents the isotype control, and blue represents HSP105 Antibody staining. A clear fluorescence shift is observed with the HSP105 Antibody compared with the isotype control, demonstrating detection of HSPH1 in human A431 cells.

HSP105 Antibody Human MCF-7 Breast Cancer Cell IF. Immunofluorescence staining of FFPE human MCF-7 breast cancer cells was performed with HSP105 Antibody, also called HSP70 Co-Chaperone Antibody (green), with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by boiling in 10 mM citrate buffer, pH 6, for 20 min followed by cooling. HSP105 staining is observed predominantly in the cytoplasmic compartment. This HSP105 Antibody demonstrates HSPH1 localization in human MCF-7 cells and supports studies of HSP70-associated chaperone activity and proteostasis.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1
Clone Name	3D10
Purity	Affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt	Q92598
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml Immunofluorescence : 2-4ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This HSP105 Antibody / HSP70 Co-Chaperone Antibody is available for research use only.
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Description

HSP105 Antibody / HSP70 Co-Chaperone Antibody recognizes heat shock protein 105, a large molecular chaperone encoded by the HSPH1 gene. HSP105 belongs to the HSP110/HSPH family of heat shock proteins and is closely associated with cellular protein quality control and stress responses. Unlike classical HSP70 proteins, HSP105 functions prominently as a co-chaperone and nucleotide exchange factor for HSP70-family proteins, helping regulate the chaperone cycle responsible for maintaining protein folding and proteostasis.

HSP105 interacts with HSP70 and related chaperones to promote the release of ADP from HSP70, facilitating nucleotide exchange and progression through the HSP70 protein-folding cycle. Through this activity, HSP105 contributes to the handling of unfolded and misfolded proteins and helps protect cells against proteotoxic stress. Members of the HSP110 family can also cooperate with HSP70 machinery in protein disaggregation, providing an important mechanism for recovering proteins from stress-induced aggregates and maintaining cellular protein homeostasis.

Expression of HSP105 can increase in response to heat shock and other forms of cellular stress. Its molecular chaperone activity has therefore made HSP105 an important target for studies of stress adaptation, protein folding, protein aggregation and proteostasis. An HSP105 Antibody can be useful for investigating changes in HSPH1 expression under conditions that challenge protein homeostasis and for examining the relationship between HSP105 and the broader HSP70 chaperone network.

HSP105 is also studied in cancer biology, where altered expression of molecular chaperones can support cellular survival under the stressful conditions associated with tumor growth. Elevated HSP105 expression has been reported in multiple malignancies, and research has examined its relationships with tumor cell survival, proliferation, stress resistance and treatment response. These observations have generated interest in HSP105 as a marker of cellular stress adaptation and as a component of chaperone networks involved in cancer-associated proteostasis.

NSJ Bioreagents offers mouse monoclonal HSP105 Antibody clone 3D10 for research into HSPH1 expression and molecular chaperone biology. An HSP70 Co-Chaperone Antibody can support studies of HSP70-associated protein folding, nucleotide exchange, proteostasis, cellular stress responses and cancer biology.

Researchers investigating HSP105 within cellular stress and protein homeostasis pathways can explore our Signal Transduction Antibodies page for additional reagents targeting proteins involved in stress-responsive cellular signaling.

Application Notes

Optimal dilution of the HSP105 Antibody / HSP70 Co-Chaperone Antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids Y653-D858) was used as the immunogen for the HSP105 antibody.

Storage

After reconstitution, the HSP105 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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HSP105 Antibody / HSP70 Co-Chaperone Antibody [clone 3D10] (RQ5517)

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