HSP10 Antibody / HSPE1 (R30887)

Western blot testing of HSP10 antibody and rat samples 1: thymus; 2: brain; 3: ovary; 4: testis; and human samples 5: A431; 6: A549; 7: MCF-7; 8: MM231; 9: HeLa cell lysate. Expected molecular weight: ~10 kDa.

IHC-P: HSP10 antibody testing of human intestinal cancer tissue

ICC testing of HSP10 antibody and A549 cells

IHC-P: HSP10 antibody testing of mouse intestine tissue

IHC staininging of FFPE rat intestinal tissue with HSP10 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of FFPE human intestinal cancer with HSP10 antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of FFPE human intestinal cancer with HSP10 antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH8 EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human lung cancer with HSP10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human HepG2 cells with HSP10 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunofluorescent staining of FFPE human HeLa cells with HSP10 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human A431, 2) human A549, 3) rat heart and 4) mouse heart lysate with HSP10 antibody. Expected molecular weight: ~10 kDa.

Flow cytometry testing of human HepG2 cells with HSP10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP10 antibody.