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Home >> Antibodies >> HSD3B1/2 Antibody

HSD3B1/2 Antibody [clone 3F3C/7F9C8] (RQ6736)

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  Catalog No Formulation Size Price (USD)  
Image RQ6736 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with HSD3B1/2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with HSD3B1/2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human placenta, 2) rat testis and 3) mouse testis tissue lysate with HSD3B1/2 antibody. Predicted molecular weight ~42 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 3F3C/7F9C8
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P14060
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Limitations This HSD3B1/2 antibody is available for research use only.
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Description

HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.

Application Notes

Optimal dilution of the HSD3B1/2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q17-Q373) was used as the immunogen for the HSD3B1/2 antibody.

Storage

After reconstitution, the HSD3B1/2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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