HSD17B1 Antibody (R30694)

HSD17B1 Antibody Multi-Species WB. Western blot analysis of human T-47D, human 293T, and rat C6 cell lysates using HSD17B1 Antibody demonstrates a prominent immunoreactive band at approximately 35 kDa, consistent with the predicted molecular weight of Hydroxysteroid 17-Beta Dehydrogenase 1 (HSD17B1). HSD17B1 is a steroidogenic enzyme that catalyzes the conversion of estrone to biologically active estradiol and plays a central role in estrogen biosynthesis and steroid hormone metabolism. Comparable band detection across human and rat cell lysates supports recognition of HSD17B1 in multiple species. Minor lower molecular weight bands are observed in the 293T lysate and may represent degradation products, alternatively processed forms, or non-specific antibody reactivity.

HSD17B1 Antibody Human Placenta IHC. Immunohistochemistry staining of FFPE human placenta tissue using HSD17B1 Antibody demonstrates distinct membranous and cytoplasmic HRP-DAB brown staining within trophoblastic cell populations lining placental villous structures. The staining pattern is consistent with expression of Hydroxysteroid 17-Beta Dehydrogenase 1 (HSD17B1), a steroidogenic enzyme that catalyzes the conversion of estrone to biologically active estradiol and plays a key role in estrogen biosynthesis during pregnancy. Prominent placental staining is consistent with the established involvement of HSD17B1 in steroid hormone metabolism, endocrine regulation, and maintenance of reproductive tissue function. Required HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes before testing.

HSD17B1 Antibody Human HeLa Cell ICC. Immunocytochemistry staining of FFPE human HeLa cells using HSD17B1 Antibody demonstrates strong cytoplasmic HRP-DAB brown staining throughout the majority of cellular populations. The staining pattern is consistent with expression of Hydroxysteroid 17-Beta Dehydrogenase 1 (HSD17B1), a steroidogenic enzyme that catalyzes the conversion of estrone to biologically active estradiol and contributes to regulation of estrogen biosynthesis and steroid hormone metabolism. Predominantly cytoplasmic localization is consistent with the established intracellular enzymatic function of HSD17B1 in hormone-responsive cellular pathways. Required HIER: steam sections in pH 6 citrate buffer for 20 minutes before testing.

HSD17B1 Antibody Rat Ovary IHC. Immunohistochemistry staining of FFPE rat ovary tissue using HSD17B1 Antibody demonstrates strong cytoplasmic HRP-DAB brown staining within ovarian follicular and steroidogenic cell populations surrounding developing follicles. The staining pattern is consistent with expression of Hydroxysteroid 17-Beta Dehydrogenase 1 (HSD17B1), a key enzyme involved in estrogen biosynthesis that catalyzes the conversion of estrone to biologically active estradiol. Prominent ovarian staining is consistent with the established role of HSD17B1 in steroid hormone metabolism, reproductive physiology, follicular development, and endocrine regulation. Required HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes before testing.

HSD17B1 Antibody Rat Liver IHC. Immunohistochemistry staining of FFPE rat liver tissue using HSD17B1 Antibody demonstrates widespread granular cytoplasmic HRP-DAB brown staining throughout hepatocyte populations. The staining pattern is consistent with expression of Hydroxysteroid 17-Beta Dehydrogenase 1 (HSD17B1), a steroidogenic enzyme involved in estrogen biosynthesis and steroid hormone metabolism. Prominent hepatic staining is consistent with the important role of the liver in steroid hormone processing, metabolic regulation, and maintenance of endocrine homeostasis. Required HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes before testing.