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Home >> Antibodies >> HPRT1 Antibody / HGPRT1

HPRT1 Antibody / HGPRT1 (F54880)

  Catalog No Formulation Size Price (USD)  
Image F54880-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54880-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with HPRT1 antibody.
Western blot testing of human HeLa cell lysate with HPRT1 antibody. Predicted molecular weight ~25 kDa.
IHC testing of FFPE mouse brain tissue with HPRT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with HPRT1 antibody; Blue=isotype control, Green= HPRT1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P00492
Localization Cytoplasmic
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:10-1:50
Western blot : 1:500-1:1000
Limitations This HPRT1 antibody is available for research use only.
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Description

Hypoxanthine-guanine phosphoribosyltransferase is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.

Application Notes

The stated application concentrations are suggested starting points. Titration of the HPRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 150-178 from the human protein was used as the immunogen for the HPRT1 antibody.

Storage

Aliquot the HPRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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