HP Antibody HP/3831 / Haptoglobin Blood Protein Antibody [clone HP/3831] (V4442)

Protein Microarray Validation of HP Antibody HP/3831. Protein microarray analysis of HP Antibody clone HP/3831 using a HuProt array containing more than 19,000 full-length human proteins identifies HP as the dominant binding target. HP ranks first with a Z-score of approximately 39.7 and an S-score of approximately 37.7, showing strong separation from the next-highest array signal. The Z-score represents binding signal relative to the mean signal across the array, while the S-score represents separation between the intended target and the next-highest protein signal.

Purified HP Antibody HP/3831 SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free HP Antibody clone HP/3831 under reducing (R) and non-reducing (NR) conditions. The reduced antibody produces the expected immunoglobulin heavy and light chain bands at approximately 50 and 25 kDa, while the non-reduced antibody migrates at approximately 150 kDa, supporting antibody integrity and purity.

HP Antibody HP/3831 Staining of Human Liver IHC. Immunohistochemistry testing of FFPE human liver tissue with HP Antibody clone HP/3831 shows strong, widespread granular brown cytoplasmic staining of hepatocytes, consistent with hepatic haptoglobin expression. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

HP Antibody HP/3831 Detection Across Mammalian Liver Tissues WB. Western blot testing of human, mouse, rat, hamster, guinea pig and rabbit liver tissue lysates with HP Antibody clone HP/3831 shows a prominent band at approximately 24 kDa in human, rat and hamster liver, while little to no corresponding signal is evident in mouse, guinea pig or rabbit liver under the tested conditions. Haptoglobin is synthesized as an approximately 45 kDa precursor that undergoes proteolytic processing into alpha and beta chains; the observed band is consistent with the processed haptoglobin beta chain, whose apparent molecular weight can also be influenced by glycosylation.

HP Antibody HP/3831 Detection in Human Liver WB. Western blot testing of human liver tissue lysate with HP Antibody clone HP/3831 shows a strong, discrete band at approximately 24 kDa. Haptoglobin is synthesized as an approximately 45 kDa precursor that undergoes proteolytic processing into alpha and beta chains; the observed band is consistent with the processed haptoglobin beta chain, whose apparent molecular weight can also be influenced by glycosylation.