HOXA3 Antibody / Homeobox Protein Hox-A3 Antibody (F55048)

HOXA3 Antibody Human Testis IHC. IHC staining of FFPE human testis tissue with HOXA3 antibody demonstrated predominantly nuclear staining in germ cells within the seminiferous tubules, consistent with the localization of HOXA3 as a homeobox transcription factor that regulates developmental gene expression and cellular differentiation. Although HOXA3 is best known for its essential role in embryonic patterning and organogenesis, continued expression in adult reproductive tissues supports functions in germ cell development, tissue homeostasis, and reproductive biology. The nuclear staining pattern is consistent with the transcriptional regulatory activity of HOXA3. HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes followed by cooling prior to staining. These results support the utility of HOXA3 Antibody for studies of developmental biology, reproductive biology, transcriptional regulation, and stem cell function.

HOXA3 Antibody Human HL60 WB. Western blot analysis of HOXA3 expression was performed using anti-HOXA3 antibody in human HL60 cell lysate. HOXA3 is a homeobox transcription factor that regulates developmental gene expression, embryonic patterning, tissue specification, and cellular differentiation during vertebrate development. A specific immunoreactive band is detected at approximately 46 kDa, consistent with the predicted molecular weight of HOXA3. Expression in HL60 promyelocytic leukemia cells is consistent with the recognized involvement of HOXA3 in hematopoietic development and the dysregulation of HOX transcription factors in leukemia. These results support the utility of HOXA3 Antibody for studies of developmental transcriptional regulation, hematopoiesis, stem cell biology, and cancer research.

HOXA3 Antibody Human HL60 Flow Cytometry. Flow cytometric analysis of human HL60 cells using HOXA3 antibody demonstrated a clear rightward shift in fluorescence intensity (green) compared with the isotype control (blue), indicating specific detection of HOXA3 expression. HOXA3 is a homeobox transcription factor that regulates developmental gene expression, hematopoietic differentiation, and cellular lineage specification. Expression in HL60 promyelocytic leukemia cells is consistent with the established role of HOXA3 and other HOX family members in hematopoiesis and leukemia biology. These results support the utility of HOXA3 Antibody for flow cytometric analysis of HOXA3 expression in studies of developmental transcriptional regulation, stem cell biology, hematopoiesis, and cancer research.