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Home >> Antibodies >> Homogentisate 1,2-dioxygenase Antibody / HGD

Homogentisate 1,2-dioxygenase Antibody / HGD [clone 2F11E1] (RQ6996)

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Image RQ6996 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human liver cancer tissue with Homogentisate 1,2-dioxygenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer tissue with Homogentisate 1,2-dioxygenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human HCCP cell lysate with Homogentisate 1,2-dioxygenase antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human Caco-2 cells with Homogentisate 1,2-dioxygenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Homogentisate 1,2-dioxygenase antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 2F11E1
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q93099
Localization Cytoplasmic
Applications Western blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Homogentisate 1,2-dioxygenase antibody is available for research use only.
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Description

The HGD gene encodes homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the catabolism of phenylalanine and tyrosine. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. This gene is mapped to chromosome 3q21-q23 by a preliminary PCR screen of hamster/human somatic cell hybrid genomic DNA samples and by fluorescence in situ hybridization.

Application Notes

Optimal dilution of the Homogentisate 1,2-dioxygenase antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids D374-N445) was used as the immunogen for the Homogentisate 1,2-dioxygenase antibody.

Storage

After reconstitution, the Homogentisate 1,2-dioxygenase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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