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Home >> Antibodies >> HNF4A Antibody

HNF4A Antibody (R31628)

  Catalog No Formulation Size Price (USD)  
R31628 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 299
Western blot testing of HNF4A antibody and Lane 1: rat liver; 2: (r) kidney; 3: (r) RH35; 4: human HeLa; 5: (h) HEPG2 lysate. Predicted molecular weight ~50 kDa.
Western blot testing of HNF4A antibody and recombinant human protein (0.5ng)
IHC-P: HNF4A antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: HNF4A antibody testing of mouse kidney tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of rat kidney tissue. HIER: steamed with pH6 citrate buffer.
IHC-F: HNF4A antibody testing of mouse liver tissue.
IHC-F: HNF4A antibody testing of rat kidney tissue.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P41235
Localization Predominantly nuclear, present in cytoplasm in cells with high HNF4A expression
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
IHC (Frozen) : 0.5-1ug/ml
Limitations This HNF4A antibody is available for research use only.
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Hepatocyte nuclear factor 4 alpha, also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is a nuclear transcription factor that binds DNA as a homodimer. HNF4A controls the expression of several genes, including HNF1A, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.


Human partial recombinant protein (AA 164-474) was used as the immunogen for this HNF4A antibody.


After reconstitution, the HNF4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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