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Home >> Antibodies >> HNF4A Antibody

HNF4A Antibody (R31628)

  Catalog No Formulation Size Price (USD)  
Image R31628 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
IHC staining of FFPE human intestinal cancer with HNF4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with HNF4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with HNF4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen mouse kidney with HNF4A antibody.
IHC staining of FFPE rat liver with HNF4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen rat liver with HNF4A antibody.
Western blot testing of human HepG2 cell lysate wiht HNF4A antibody. Predicted molecular weight ~50 kDa.
IF/ICC staining of FFPE human HEPG2 cells with HNF4A antibody (green) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human HepG2 cells with HNF4A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HNF4A antibody.
IHC staining of FFPE mouse intestine with HNF4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P41235
Localization Predominantly nuclear, present in cytoplasm in cells with high HNF4A expression
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
IHC (Frozen) : 1-2ug/ml
IF/ICC (FFPE) : 2-4ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This HNF4A antibody is available for research use only.
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Description

Hepatocyte nuclear factor 4 alpha, also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is a nuclear transcription factor that binds DNA as a homodimer. HNF4A controls the expression of several genes, including HNF1A, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 164-474) was used as the immunogen for this HNF4A antibody.

Storage

After reconstitution, the HNF4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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