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Home >> Antibodies >> HNF4A Antibody

HNF4A Antibody (F48198)

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  Catalog No Formulation Size Price (USD)  
Image F48198-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48198-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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HNF4A antibody western blot analysis in 1) 293, 2) HepG2 cell line and 3) rat liver tissue lysate. Predicted molecular weight ~50 kDa.
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HNF4A antibody western blot analysis in 1) 293, 2) HepG2 cell line and 3) rat liver tissue lysate. Predicted molecular weight ~50 kDa.
HNF4A antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue.
Confocal immunofluorescent analysis of HNF4A antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
HNF4A antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human, Rat
Predicted Reactivity Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P41235
Localization Predominantly nuclear, present in cytoplasm in cells with high HNF4A expression
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This HNF4A antibody is available for research use only.
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Description

Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProt]

Application Notes

Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 281-312 from the human protein was used as the immunogen for this HNF4A antibody.

Storage

Aliquot the HNF4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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