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Home >> Antibodies >> HNF1A Antibody

HNF1A Antibody [clone HPTF1A-1] (V7409)

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  Catalog No Formulation Size Price (USD)  
Image V7409-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7409-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7409SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7409IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human small intestine with HNF1A antibody (clone HPTF1A-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human small intestine with HNF1A antibody (clone HPTF1A-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name HPTF1A-1
Purity Protein G affinity chromatography
UniProt P20823
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This HNF1A antibody is available for research use only.
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Description

HNF1A is a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the HNF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 214-339 from the human protein was used as the immunogen for the HNF1A antibody.

Storage

Store the HNF1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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